This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6reg
From Proteopedia
(Difference between revisions)
m (Protected "6reg" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Pizza6-S with Zn2+== | |
| + | <StructureSection load='6reg' size='340' side='right'caption='[[6reg]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6reg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6REG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6REG FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6reg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6reg OCA], [http://pdbe.org/6reg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6reg RCSB], [http://www.ebi.ac.uk/pdbsum/6reg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6reg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We developed an artificial hydrolase based on the symmetrical Pizza6 beta-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical beta-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials. | ||
| - | + | Artificial beta-propeller protein-based hydrolases.,Clarke DE, Noguchi H, Gryspeerdt JAG, De Feyter S, Voet ARD Chem Commun (Camb). 2019 Jul 23;55(60):8880-8883. doi: 10.1039/c9cc04388h. PMID:31321399<ref>PMID:31321399</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6reg" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Clarke, D E]] | ||
| + | [[Category: Feyter, S D]] | ||
| + | [[Category: Gryspeerdt, J L]] | ||
[[Category: Noguchi, H]] | [[Category: Noguchi, H]] | ||
| - | [[Category: | + | [[Category: Voet, A R.D]] |
| - | [[Category: | + | [[Category: Artificial beta-propeller protein]] |
| - | [[Category: | + | [[Category: Computational design]] |
| - | [[Category: | + | [[Category: De novo protein]] |
| + | [[Category: Metalloprotein]] | ||
| + | [[Category: Pizza]] | ||
| + | [[Category: Zinc]] | ||
Revision as of 06:32, 31 July 2019
Crystal structure of Pizza6-S with Zn2+
| |||||||||||
