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6reh
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Pizza6-S with Cu2+== | |
| + | <StructureSection load='6reh' size='340' side='right'caption='[[6reh]], [[Resolution|resolution]] 1.73Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6reh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6REH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6REH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6reh OCA], [http://pdbe.org/6reh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6reh RCSB], [http://www.ebi.ac.uk/pdbsum/6reh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6reh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We developed an artificial hydrolase based on the symmetrical Pizza6 beta-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical beta-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials. | ||
| - | + | Artificial beta-propeller protein-based hydrolases.,Clarke DE, Noguchi H, Gryspeerdt JAG, De Feyter S, Voet ARD Chem Commun (Camb). 2019 Jul 23;55(60):8880-8883. doi: 10.1039/c9cc04388h. PMID:31321399<ref>PMID:31321399</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6reh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Clarke, D E]] | ||
| + | [[Category: Feyter, S D]] | ||
| + | [[Category: Gryspeerdt, J L]] | ||
[[Category: Noguchi, H]] | [[Category: Noguchi, H]] | ||
| - | [[Category: | + | [[Category: Voet, A R.D]] |
| - | [[Category: | + | [[Category: Artificial beta-propeller protein]] |
| - | [[Category: | + | [[Category: Computational design]] |
| - | [[Category: | + | [[Category: Copper]] |
| + | [[Category: De novo protein]] | ||
| + | [[Category: Metalloprotein]] | ||
| + | [[Category: Pizza]] | ||
Revision as of 06:32, 31 July 2019
Crystal structure of Pizza6-S with Cu2+
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