This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

6od1

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 06:20, 12 June 2019

IraD-bound to RssB D58P variant

Structural highlights

6od1 is a 2 chain structure with sequence from Ecoli and Escherichia coli 907672. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	rssB, HMPREF1595_01509 (Escherichia coli 907672), iraD, yjiD, b4326, JW5782 (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[V0SNG0_ECOLX] Regulates the turnover of the sigma S factor (RpoS) by promoting its proteolysis in exponentially growing cells. Acts by binding and delivering RpoS to the ClpXP protease. RssB is not co-degraded with RpoS, but is released from the complex and can initiate a new cycle of RpoS recognition and degradation.[HAMAP-Rule:MF_00958] [IRAD_ECOLI] Inhibits RpoS proteolysis by regulating RssB activity, thereby increasing the stability of the sigma stress factor RpoS during oxidative stress. Its effect on RpoS stability is due to its interaction with RssB, which probably blocks the interaction of RssB with RpoS, and the consequent delivery of the RssB-RpoS complex to the ClpXP protein degradation pathway.^[1]

Publication Abstract from PubMed

The stationary phase promoter specificity subunit sigma(S) (RpoS) is delivered to the ClpXP machinery for degradation dependent on the adaptor RssB. This adaptor-specific degradation of sigma(S) provides a major point for regulation and transcriptional reprogramming during the general stress response. RssB is an atypical response regulator and the only known ClpXP adaptor that is inhibited by multiple but dissimilar antiadaptors (IraD, IraP, and IraM). These are induced by distinct stress signals and bind to RssB in poorly understood manners to achieve stress-specific inhibition of sigma(S) turnover. Here we present the first crystal structure of RssB bound to an antiadaptor, the DNA damage-inducible IraD. The structure reveals that RssB adopts a compact closed architecture with extensive interactions between its N-terminal and C-terminal domains. The structural data, together with mechanistic studies, suggest that RssB plasticity, conferred by an interdomain glutamate-rich flexible linker, is critical for regulation of sigma(S) degradation. Structural modulation of interdomain linkers may thus constitute a general strategy for tuning response regulators.

Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor.,Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM Genes Dev. 2019 Apr 11. pii: gad.320168.118. doi: 10.1101/gad.320168.118. PMID:30975721^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bougdour A, Cunning C, Baptiste PJ, Elliott T, Gottesman S. Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors. Mol Microbiol. 2008 Apr;68(2):298-313. PMID:18383615
↑ Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM. Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor. Genes Dev. 2019 Apr 11. pii: gad.320168.118. doi: 10.1101/gad.320168.118. PMID:30975721 doi:http://dx.doi.org/10.1101/gad.320168.118

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6od1"

@@ Line 3: / Line 3: @@
 <StructureSection load='6od1' size='340' side='right'caption='[[6od1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6od1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OD1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OD1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6od1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Escherichia_coli_907672 Escherichia coli 907672]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OD1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OD1 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6od1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6od1 OCA], [http://pdbe.org/6od1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6od1 RCSB], [http://www.ebi.ac.uk/pdbsum/6od1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6od1 ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rssB, HMPREF1595_01509 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1268982 Escherichia coli 907672]), iraD, yjiD, b4326, JW5782 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6od1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6od1 OCA], [http://pdbe.org/6od1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6od1 RCSB], [http://www.ebi.ac.uk/pdbsum/6od1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6od1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Ecoli]]
+[[Category: Escherichia coli 907672]]
 [[Category: Large Structures]]
 [[Category: Deaconescu, A M]]

6od1

From Proteopedia

Revision as of 06:20, 12 June 2019

IraD-bound to RssB D58P variant

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox