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| | <StructureSection load='6g4h' size='340' side='right'caption='[[6g4h]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6g4h' size='340' side='right'caption='[[6g4h]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6g4h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G4H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G4H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6g4h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G4H FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6g49|6g49]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tgpA, PA2873 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g4h OCA], [https://pdbe.org/6g4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g4h RCSB], [https://www.ebi.ac.uk/pdbsum/6g4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g4h ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g4h OCA], [http://pdbe.org/6g4h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g4h RCSB], [http://www.ebi.ac.uk/pdbsum/6g4h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g4h ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TGPA_PSEAE TGPA_PSEAE]] Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.<ref>PMID:23209712</ref> | + | [https://www.uniprot.org/uniprot/TGPA_PSEAE TGPA_PSEAE] Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.<ref>PMID:23209712</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Protein-glutamine gamma-glutamyltransferase]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Pseae]]
| + | [[Category: Mastrangelo E]] |
| - | [[Category: Mastrangelo, E]] | + | [[Category: Milani M]] |
| - | [[Category: Milani, M]] | + | [[Category: Uruburu M]] |
| - | [[Category: Uruburu, M]] | + | |
| - | [[Category: Essential bacterial protein]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
TGPA_PSEAE Displays transglutaminase activity (TGase) in vitro. Plays a critical role in the viability of P.aeruginosa. Might contribute to an essential function linked to the cell wall.[1]
Publication Abstract from PubMed
Pseudomonas aeruginosa is an opportunistic pathogen associated with severe diseases, such as cystic fibrosis. During an extensive search for novel essential genes, we identified tgpA (locus PA2873) in P. aeruginosa PAO1, as a gene playing a critical role in bacterial viability. TgpA, the translated protein, is an internal membrane protein with a periplasmic soluble domain, predicted to be endowed with a transglutaminase-like fold, hosting the Cys404, His448, and Asp464 triad. We report here that Cys404 mutation hampers the essential role of TgpA in granting P. aeruginosa viability. Moreover, we present the crystal structure of the TgpA periplasmic domain at 1.6A resolution as a first step towards structure-activity analysis of a new potential target for the discovery of antibacterial compounds.
Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa.,Uruburu M, Mastrangelo E, Bolognesi M, Ferrara S, Bertoni G, Milani M J Struct Biol. 2019 Mar 1;205(3):18-25. doi: 10.1016/j.jsb.2018.12.004. Epub 2018, Dec 30. PMID:30599211[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Milani A, Vecchietti D, Rusmini R, Bertoni G. TgpA, a protein with a eukaryotic-like transglutaminase domain, plays a critical role in the viability of Pseudomonas aeruginosa. PLoS One. 2012;7(11):e50323. doi: 10.1371/journal.pone.0050323. Epub 2012 Nov 27. PMID:23209712 doi:http://dx.doi.org/10.1371/journal.pone.0050323
- ↑ Uruburu M, Mastrangelo E, Bolognesi M, Ferrara S, Bertoni G, Milani M. Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa. J Struct Biol. 2019 Mar 1;205(3):18-25. doi: 10.1016/j.jsb.2018.12.004. Epub 2018, Dec 30. PMID:30599211 doi:http://dx.doi.org/10.1016/j.jsb.2018.12.004
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