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| | <StructureSection load='4qcf' size='340' side='right'caption='[[4qcf]], [[Resolution|resolution]] 2.26Å' scene=''> | | <StructureSection load='4qcf' size='340' side='right'caption='[[4qcf]], [[Resolution|resolution]] 2.26Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qcf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QCF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qcf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._NG-27 Bacillus sp. NG-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QCF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2f8q|2f8q]], [[2fgl|2fgl]], [[4qce|4qce]], [[4qdm|4qdm]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qcf OCA], [https://pdbe.org/4qcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qcf RCSB], [https://www.ebi.ac.uk/pdbsum/4qcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qcf ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qcf OCA], [http://pdbe.org/4qcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qcf RCSB], [http://www.ebi.ac.uk/pdbsum/4qcf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qcf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus sp. ng-27]] | + | [[Category: Bacillus sp. NG-27]] |
| - | [[Category: Endo-1,4-beta-xylanase]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bhardwaj, A]] | + | [[Category: Bhardwaj A]] |
| - | [[Category: Mahanta, P]] | + | [[Category: Mahanta P]] |
| - | [[Category: Ramakumar, S]] | + | [[Category: Ramakumar S]] |
| - | [[Category: Reddy, V S]] | + | [[Category: Reddy VS]] |
| - | [[Category: Gh10 xylanase]]
| + | |
| - | [[Category: Glycosyl hydrolase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
O30700_9BACI
Publication Abstract from PubMed
Although several factors have been suggested to contribute to thermostability, the stabilization strategies used by proteins are still enigmatic. Studies on a recombinant xylanase from Bacilllus sp. NG-27 (RBSX), which has the ubiquitous (beta/alpha)8 -triosephosphate isomerase barrel fold, showed that just a single mutation, V1L, although not located in any secondary structural element, markedly enhanced the stability from 70 degrees C to 75 degrees C without loss of catalytic activity. Conversely, the V1A mutation at the same position decreased the stability of the enzyme from 70 degrees C to 68 degrees C. To gain structural insights into how a single extreme N-terminus mutation can markedly influence the thermostability of the enzyme, we determined the crystal structure of RBSX and the two mutants. On the basis of computational analysis of their crystal structures, including residue interaction networks, we established a link between N-terminal to C-terminal contacts and RBSX thermostability. Our study reveals that augmenting N-terminal to C-terminal noncovalent interactions is associated with enhancement of the stability of the enzyme. In addition, we discuss several lines of evidence supporting a connection between N-terminal to C-terminal noncovalent interactions and protein stability in different proteins. We propose that the strategy of mutations at the termini could be exploited with a view to modulate stability without compromising enzymatic activity, or in general, protein function in diverse folds where N and C termini are in close proximity. DATABASE: The coordinates of RBSX, V1A and V1L have been deposited in the PDB database under the accession numbers 4QCE, 4QCF, and 4QDM, respectively.
Structural insights into N-terminal to C-terminal interactions and implications for thermostability of a (beta/alpha)8-triosephosphate isomerase barrel enzyme.,Mahanta P, Bhardwaj A, Kumar K, Reddy VS, Ramakumar S FEBS J. 2015 Sep;282(18):3543-55. doi: 10.1111/febs.13355. Epub 2015 Jul 15. PMID:26102498[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mahanta P, Bhardwaj A, Kumar K, Reddy VS, Ramakumar S. Structural insights into N-terminal to C-terminal interactions and implications for thermostability of a (beta/alpha)8-triosephosphate isomerase barrel enzyme. FEBS J. 2015 Sep;282(18):3543-55. doi: 10.1111/febs.13355. Epub 2015 Jul 15. PMID:26102498 doi:http://dx.doi.org/10.1111/febs.13355
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