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| | <StructureSection load='4qmd' size='340' side='right'caption='[[4qmd]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='4qmd' size='340' side='right'caption='[[4qmd]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4qmd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QMD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4qmd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QMD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EVPL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmd OCA], [https://pdbe.org/4qmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qmd RCSB], [https://www.ebi.ac.uk/pdbsum/4qmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmd OCA], [http://pdbe.org/4qmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qmd RCSB], [http://www.ebi.ac.uk/pdbsum/4qmd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EVPL_HUMAN EVPL_HUMAN]] Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. | + | [https://www.uniprot.org/uniprot/EVPL_HUMAN EVPL_HUMAN] Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Al-Jassar, C]] | + | [[Category: Al-Jassar C]] |
| - | [[Category: Chidgey, M]] | + | [[Category: Chidgey M]] |
| - | [[Category: Fogl, C]] | + | [[Category: Fogl C]] |
| - | [[Category: Jeeves, M]] | + | [[Category: Jeeves M]] |
| - | [[Category: Knowles, T J]] | + | [[Category: Knowles TJ]] |
| - | [[Category: Mohammed, F]] | + | [[Category: Mohammed F]] |
| - | [[Category: Odinstova, E]] | + | [[Category: Odinstova E]] |
| - | [[Category: Overduin, M]] | + | [[Category: Overduin M]] |
| - | [[Category: Rodriguez-Zamora, P]] | + | [[Category: Rodriguez-Zamora P]] |
| - | [[Category: White, S A]] | + | [[Category: White SA]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Cell junction]]
| + | |
| - | [[Category: Cornified envelope]]
| + | |
| - | [[Category: Cytoskeleton]]
| + | |
| - | [[Category: Desmosome]]
| + | |
| - | [[Category: Envoplakin]]
| + | |
| - | [[Category: Epidermal permeability barrier]]
| + | |
| - | [[Category: Intermediate filament]]
| + | |
| - | [[Category: Keratinocyte]]
| + | |
| - | [[Category: Paraneoplastic pemphigus]]
| + | |
| - | [[Category: Periplakin]]
| + | |
| - | [[Category: Plakin protein]]
| + | |
| - | [[Category: Plakin repeat domain]]
| + | |
| - | [[Category: Terminal differentiation]]
| + | |
| - | [[Category: Vimentin]]
| + | |
| Structural highlights
Function
EVPL_HUMAN Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
Publication Abstract from PubMed
Plakin proteins form critical connections between cell junctions and the cytoskeleton; their disruption within epithelial and cardiac muscle cells cause skin-blistering diseases and cardiomyopathies. Envoplakin has a single plakin repeat domain (PRD) which recognizes intermediate filaments through an unresolved mechanism. Herein we report the crystal structure of envoplakin's complete PRD fold, revealing binding determinants within its electropositive binding groove. Four of its five internal repeats recognize negatively charged patches within vimentin via five basic determinants that are identified by nuclear magnetic resonance spectroscopy. Mutations of the Lys1901 or Arg1914 binding determinants delocalize heterodimeric envoplakin from intracellular vimentin and keratin filaments in cultured cells. Recognition of vimentin is abolished when its residues Asp112 or Asp119 are mutated. The latter slot intermediate filament rods into basic PRD domain grooves through electrosteric complementarity in a widely applicable mechanism. Together this reveals how plakin family members form dynamic linkages with cytoskeletal frameworks.
Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin.,Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M Nat Commun. 2016 Mar 3;7:10827. doi: 10.1038/ncomms10827. PMID:26935805[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M. Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin. Nat Commun. 2016 Mar 3;7:10827. doi: 10.1038/ncomms10827. PMID:26935805 doi:http://dx.doi.org/10.1038/ncomms10827
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