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4rfs

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Revision as of 06:53, 2 March 2023

Structure of a pantothenate energy coupling factor transporter

Structural highlights

4rfs is a 4 chain structure with sequence from Levilactobacillus brevis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ECFA2_LEVBA ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.[HAMAP-Rule:MF_01710]

Publication Abstract from PubMed

Energy-coupling factor (ECF) transporters are a unique group of ATP-binding cassette (ABC) transporters responsible for micronutrient uptake from the environment. Each ECF transporter is composed of an S component (or EcfS protein) and T/A/A' components (or EcfT/A/A' proteins; ECF module). Among the group II ECF transporters, several EcfS proteins share one ECF module; however, the underlying mechanism remains unknown. Here we report the structure of a group II ECF transporter-pantothenate transporter from Lactobacillus brevis (LbECF-PanT), which shares the ECF module with the folate and hydroxymethylpyrimidine transporters (LbECF-FolT and LbECF-HmpT). Structural and mutational analyses revealed the residues constituting the pantothenate-binding pocket. We found that although the three EcfS proteins PanT, FolT, and HmpT are dissimilar in sequence, they share a common surface area composed of the transmembrane helices 1/2/6 (SM1/2/6) to interact with the coupling helices 2/3 (CH2/3) of the same EcfT. CH2 interacts mainly with SM1 via hydrophobic interactions, which may modulate the sliding movement of EcfS. CH3 binds to a hydrophobic surface groove formed by SM1, SM2, and SM6, which may transmit the conformational changes from EcfA/A' to EcfS. We also found that the residues at the intermolecular surfaces in LbECF-PanT are essential for transporter activity, and that these residues may mediate intermolecular conformational transmission and/or affect transporter complex stability. In addition, we found that the structure of EcfT is conformationally dynamic, which supports its function as a scaffold to mediate the interaction of the ECF module with various EcfS proteins to form different transporter complexes.

Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters.,Zhang M, Bao Z, Zhao Q, Guo H, Xu K, Wang C, Zhang P Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18560-5. doi:, 10.1073/pnas.1412246112. Epub 2014 Dec 15. PMID:25512487^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang M, Bao Z, Zhao Q, Guo H, Xu K, Wang C, Zhang P. Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters. Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18560-5. doi:, 10.1073/pnas.1412246112. Epub 2014 Dec 15. PMID:25512487 doi:http://dx.doi.org/10.1073/pnas.1412246112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4rfs"

@@ Line 3: / Line 3: @@
 <StructureSection load='4rfs' size='340' side='right'caption='[[4rfs]], [[Resolution|resolution]] 3.23&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4rfs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RFS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4rfs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis Levilactobacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RFS FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ecfA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904]), ecfA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904]), LVIS_0658 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904]), ecfT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfs OCA], [https://pdbe.org/4rfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rfs RCSB], [https://www.ebi.ac.uk/pdbsum/4rfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rfs ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfs OCA], [http://pdbe.org/4rfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rfs RCSB], [http://www.ebi.ac.uk/pdbsum/4rfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rfs ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ECFT_LACBA ECFT_LACBA]] Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). [[http://www.uniprot.org/uniprot/ECFA2_LACBA ECFA2_LACBA]] Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). [[http://www.uniprot.org/uniprot/ECFA1_LACBA ECFA1_LACBA]] Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity).
+[https://www.uniprot.org/uniprot/ECFA2_LEVBA ECFA2_LEVBA] ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.[HAMAP-Rule:MF_01710]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus casei g von freudenreich and thoni 1904]]
 [[Category: Large Structures]]
-[[Category: Bao, Z]]
+[[Category: Levilactobacillus brevis]]
-[[Category: Guo, H]]
+[[Category: Bao Z]]
-[[Category: Xu, K]]
+[[Category: Guo H]]
-[[Category: Zhang, M]]
+[[Category: Xu K]]
-[[Category: Zhang, P]]
+[[Category: Zhang M]]
-[[Category: Zhao, Q]]
+[[Category: Zhang P]]
-[[Category: Ecf]]
+[[Category: Zhao Q]]
-[[Category: Hydrolase]]
-[[Category: Transport protein]]
-[[Category: Transporter]]

4rfs

From Proteopedia

Revision as of 06:53, 2 March 2023

Structure of a pantothenate energy coupling factor transporter

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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