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| | <StructureSection load='4rlc' size='340' side='right'caption='[[4rlc]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='4rlc' size='340' side='right'caption='[[4rlc]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4rlc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RLC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4rlc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RLC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rl9|4rl9]], [[4rlb|4rlb]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rlc OCA], [https://pdbe.org/4rlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rlc RCSB], [https://www.ebi.ac.uk/pdbsum/4rlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rlc ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">oprF, PA1777 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rlc OCA], [http://pdbe.org/4rlc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rlc RCSB], [http://www.ebi.ac.uk/pdbsum/4rlc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rlc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PORF_PSEAE PORF_PSEAE]] Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium. | + | [https://www.uniprot.org/uniprot/PORF_PSEAE PORF_PSEAE] Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Porin|Porin]] | + | *[[Porin 3D structures|Porin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Basle, A]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Berg, B van den]] | + | [[Category: Basle A]] |
| - | [[Category: Zahn, M]] | + | [[Category: Zahn M]] |
| - | [[Category: Beta-barrel]] | + | [[Category: Van den Berg B]] |
| - | [[Category: Outer membrane protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
PORF_PSEAE Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
Publication Abstract from PubMed
Outer membrane (OM) beta-barrel proteins composed of 12-18 beta-strands mediate cellular entry of small molecules in Gram-negative bacteria. Small OM proteins with barrels of 10 strands or less are not known to transport small molecules. CarO (carbapenem-associated outer membrane protein) from Acinetobacter baumannii is a small OM protein that has been implicated in the uptake of ornithine and carbapenem antibiotics. Here we report crystal structures of three isoforms of CarO. The structures are very similar and show a monomeric eight-stranded barrel lacking an open channel. CarO has a substantial extracellular domain resembling a glove that contains all the divergent residues between the different isoforms. Liposome swelling experiments demonstrate that full-length CarO and a "loop-less" truncation mutant mediate small-molecule uptake at low levels but that they are unlikely to mediate passage of carbapenem antibiotics. These results are confirmed by biased molecular dynamics simulations that allowed us to quantitatively model the transport of selected small molecules.
Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacterbaumannii.,Zahn M, D'Agostino T, Eren E, Basle A, Ceccarelli M, van den Berg B J Mol Biol. 2015 Apr 3. pii: S0022-2836(15)00201-6. doi:, 10.1016/j.jmb.2015.03.016. PMID:25846137[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zahn M, D'Agostino T, Eren E, Basle A, Ceccarelli M, van den Berg B. Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacterbaumannii. J Mol Biol. 2015 Apr 3. pii: S0022-2836(15)00201-6. doi:, 10.1016/j.jmb.2015.03.016. PMID:25846137 doi:http://dx.doi.org/10.1016/j.jmb.2015.03.016
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