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| | <StructureSection load='4rhb' size='340' side='right'caption='[[4rhb]], [[Resolution|resolution]] 3.35Å' scene=''> | | <StructureSection load='4rhb' size='340' side='right'caption='[[4rhb]], [[Resolution|resolution]] 3.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4rhb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4rhb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nhr|4nhr]], [[4rh8|4rh8]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhb OCA], [https://pdbe.org/4rhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rhb RCSB], [https://www.ebi.ac.uk/pdbsum/4rhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhb ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lptD, imp, ostA, yabG, b0054, JW0053 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), lptE, rlpB, b0641, JW0636 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhb OCA], [http://pdbe.org/4rhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rhb RCSB], [http://www.ebi.ac.uk/pdbsum/4rhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhb ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LPTD_ECOLI LPTD_ECOLI]] Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Contributes to n-hexane resistance.[HAMAP-Rule:MF_01411]<ref>PMID:12207697</ref> <ref>PMID:12724388</ref> <ref>PMID:16861298</ref> <ref>PMID:18424520</ref> <ref>PMID:20203010</ref> <ref>PMID:21339611</ref> <ref>PMID:2547691</ref> <ref>PMID:7811102</ref> [[http://www.uniprot.org/uniprot/LPTE_ECOLI LPTE_ECOLI]] Together with LptD, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Required for the proper assembly of LptD. Binds LPS and may serve as the LPS recognition site at the outer membrane.<ref>PMID:16861298</ref> <ref>PMID:18424520</ref> <ref>PMID:20203010</ref> | + | [https://www.uniprot.org/uniprot/LPTD_ECOLI LPTD_ECOLI] Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Contributes to n-hexane resistance.[HAMAP-Rule:MF_01411]<ref>PMID:12207697</ref> <ref>PMID:12724388</ref> <ref>PMID:16861298</ref> <ref>PMID:18424520</ref> <ref>PMID:20203010</ref> <ref>PMID:21339611</ref> <ref>PMID:2547691</ref> <ref>PMID:7811102</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Garner, R A]] | + | [[Category: Garner RA]] |
| - | [[Category: Kahne, D]] | + | [[Category: Kahne D]] |
| - | [[Category: Malojcic, G]] | + | [[Category: Malojcic G]] |
| - | [[Category: 26-stranded beta barrel and 2-layer beta sandwich]]
| + | |
| - | [[Category: Lipid binding protein]]
| + | |
| - | [[Category: Lipopolysaccharide assembly]]
| + | |
| - | [[Category: Outer membrane of gram-negative bacteria]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
LPTD_ECOLI Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Contributes to n-hexane resistance.[HAMAP-Rule:MF_01411][1] [2] [3] [4] [5] [6] [7] [8]
References
- ↑ Braun M, Silhavy TJ. Imp/OstA is required for cell envelope biogenesis in Escherichia coli. Mol Microbiol. 2002 Sep;45(5):1289-302. PMID:12207697
- ↑ Abe S, Okutsu T, Nakajima H, Kakuda N, Ohtsu I, Aono R. n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane. Microbiology. 2003 May;149(Pt 5):1265-73. PMID:12724388
- ↑ Wu T, McCandlish AC, Gronenberg LS, Chng SS, Silhavy TJ, Kahne D. Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11754-9. Epub 2006 Jul 21. PMID:16861298 doi:http://dx.doi.org/0604744103
- ↑ Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08
- ↑ Chng SS, Ruiz N, Chimalakonda G, Silhavy TJ, Kahne D. Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5363-8. doi:, 10.1073/pnas.0912872107. Epub 2010 Mar 4. PMID:20203010 doi:http://dx.doi.org/10.1073/pnas.0912872107
- ↑ Haarmann R, Ibrahim M, Stevanovic M, Bredemeier R, Schleiff E. The properties of the outer membrane localized Lipid A transporter LptD. J Phys Condens Matter. 2010 Nov 17;22(45):454124. doi:, 10.1088/0953-8984/22/45/454124. Epub 2010 Oct 29. PMID:21339611 doi:http://dx.doi.org/10.1088/0953-8984/22/45/454124
- ↑ Sampson BA, Misra R, Benson SA. Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability. Genetics. 1989 Jul;122(3):491-501. PMID:2547691
- ↑ Aono R, Negishi T, Nakajima H. Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli. Appl Environ Microbiol. 1994 Dec;60(12):4624-6. PMID:7811102
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