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2x9d

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Revision as of 08:37, 10 November 2021

Tet repressor (class D) in complex with iso-7-chlortetracycline

Structural highlights

2x9d is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. This structure supersedes the now removed PDB entry 1du7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2xgc, 2xpw, 1bjz, 2trt, 2xpv, 2vkv, 1ork, 2xge, 1a6i, 2x6o, 1qpi, 2vke, 2xgd, 2xrl, 2xpt, 2xpu, 2xb5, 1bj0, 2xps, 1bjy, 2tct, 1du7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.

Publication Abstract from PubMed

Tetracycline antibiotics and their degradation products appear in medically treated tissues, food, soil, and manure sludge in the environment. In the context of protein interactions with various tetracyclines we performed crystal structure analyses of the tetracycline repressor in complex with weak or noninducing tetracycline derivatives. Isotetracyclines are degradation products of tetracyclines, which occur under physiological conditions. The typical framework of the antibiotic is irreversibly broken at the BC-ring connection, leading to a modified orientation of the AB to the new C*D ring fragments. The shape of the zwitterionic AB-ring fragment is unchanged and still binds to the TetR recognition site in a manner comparable to the intact antibiotic but without typical Mg(2+) chelation. This work is an example that drug degradation products can still bind to specific targets and should be discussed in light of potential and critical side effects.

Recognition of drug degradation products by target proteins: isotetracycline binding to Tet repressor.,Volkers G, Petruschka L, Hinrichs W J Med Chem. 2011 Jul 28;54(14):5108-15. Epub 2011 Jul 1. PMID:21699184^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Volkers G, Petruschka L, Hinrichs W. Recognition of drug degradation products by target proteins: isotetracycline binding to Tet repressor. J Med Chem. 2011 Jul 28;54(14):5108-15. Epub 2011 Jul 1. PMID:21699184 doi:10.1021/jm200332e

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2x9d"

@@ Line 3: / Line 3: @@
 <StructureSection load='2x9d' size='340' side='right'caption='[[2x9d]], [[Resolution|resolution]] 2.34&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2x9d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1du7 1du7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X9D FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2x9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1du7 1du7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X9D FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ITC:ISO-7-CHLORTETRACYCLINE'>ITC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ITC:ISO-7-CHLORTETRACYCLINE'>ITC</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xgc|2xgc]], [[2xpw|2xpw]], [[1bjz|1bjz]], [[2trt|2trt]], [[2xpv|2xpv]], [[2vkv|2vkv]], [[1ork|1ork]], [[2xge|2xge]], [[1a6i|1a6i]], [[2x6o|2x6o]], [[1qpi|1qpi]], [[2vke|2vke]], [[2xgd|2xgd]], [[2xrl|2xrl]], [[2xpt|2xpt]], [[2xpu|2xpu]], [[2xb5|2xb5]], [[1bj0|1bj0]], [[2xps|2xps]], [[1bjy|1bjy]], [[2tct|2tct]], [[1du7|1du7]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xgc|2xgc]], [[2xpw|2xpw]], [[1bjz|1bjz]], [[2trt|2trt]], [[2xpv|2xpv]], [[2vkv|2vkv]], [[1ork|1ork]], [[2xge|2xge]], [[1a6i|1a6i]], [[2x6o|2x6o]], [[1qpi|1qpi]], [[2vke|2vke]], [[2xgd|2xgd]], [[2xrl|2xrl]], [[2xpt|2xpt]], [[2xpu|2xpu]], [[2xb5|2xb5]], [[1bj0|1bj0]], [[2xps|2xps]], [[1bjy|1bjy]], [[2tct|2tct]], [[1du7|1du7]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x9d OCA], [http://pdbe.org/2x9d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x9d RCSB], [http://www.ebi.ac.uk/pdbsum/2x9d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x9d ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x9d OCA], [https://pdbe.org/2x9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x9d RCSB], [https://www.ebi.ac.uk/pdbsum/2x9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x9d ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
+[[https://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Tetracycline repressor protein|Tetracycline repressor protein]]
+*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
 == References ==
 <references/>

2x9d

From Proteopedia

Revision as of 08:37, 10 November 2021

Tet repressor (class D) in complex with iso-7-chlortetracycline

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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