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| | <StructureSection load='5d2a' size='340' side='right'caption='[[5d2a]], [[Resolution|resolution]] 2.13Å' scene=''> | | <StructureSection load='5d2a' size='340' side='right'caption='[[5d2a]], [[Resolution|resolution]] 2.13Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5d2a]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D2A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5d2a]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D2A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PHB:P-HYDROXYBENZOIC+ACID'>PHB</scene>, <scene name='pdbligand=ZDC:3,7-ANHYDRO-2,8-DIDEOXY-L-GLYCERO-D-GLUCO-OCTONIC+ACID'>ZDC</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PHB:P-HYDROXYBENZOIC+ACID'>PHB</scene>, <scene name='pdbligand=ZDC:3,7-ANHYDRO-2,8-DIDEOXY-L-GLYCERO-D-GLUCO-OCTONIC+ACID'>ZDC</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lecB, ERS445055_01627, PA8380_17510, PAE221_03716, PAMH19_1713, YQ19_10010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d2a OCA], [https://pdbe.org/5d2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d2a RCSB], [https://www.ebi.ac.uk/pdbsum/5d2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d2a ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d2a OCA], [http://pdbe.org/5d2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d2a RCSB], [http://www.ebi.ac.uk/pdbsum/5d2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d2a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Darbre, T]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Michaud, G]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Reymond, J L]] | + | [[Category: Darbre T]] |
| - | [[Category: Stocker, A]] | + | [[Category: Michaud G]] |
| - | [[Category: Visini, R]] | + | [[Category: Reymond JL]] |
| - | [[Category: Bifunctional]] | + | [[Category: Stocker A]] |
| - | [[Category: Biofilm]] | + | [[Category: Visini R]] |
| - | [[Category: Dendrimer]]
| + | |
| - | [[Category: Lectinb]]
| + | |
| - | [[Category: Pseudomona]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
Q9HYN5_PSEAE
Publication Abstract from PubMed
Antibiotic resistance in the opportunistic pathogen Pseudomonas aeruginosa is partly caused by biofilms forming a physical barrier to antibiotic penetration. Here we focused on modifying tetravalent glycopeptide dendrimer ligands of P. aeruginosa lectins LecB or LecA to increase their biofilm inhibition activity. First heteroglycoclusters were investigated displaying one pair each of LecB specific fucosyl groups and LecA specific galactosyl groups and binding simultaneously to both lectins, one of which gave the first fully resolved crystal structure of a peptide dendrimer as LecB complex providing a structural model for dendrimer-lectin interactions (PDB ; 5D2A). Biofilm inhibition was increased by introducing additional cationic residues in these dendrimers but resulted in bactericidal effects similar to those of non-glycosylated polycationic antimicrobial peptide dendrimers. In a second approach dendrimers displaying four copies of the natural LecB ligand Lewis(a) were prepared leading to slightly stronger LecB binding and biofilm inhibition. Finally synergistic application of a LecB specific non-bactericidal antibiofilm dendrimer with the antibiotic tobramycin at sub-inhibitory concentrations of both compounds allowed effective biofilm inhibition and dispersal.
Overcoming antibiotic resistance in Pseudomonas aeruginosa biofilms using glycopeptide dendrimers.,Michaud G, Visini R, Bergmann M, Salerno G, Bosco R, Gillon E, Richichi B, Nativi C, Imberty A, Stocker A, Darbre T, Reymond JL Chem Sci. 2016 Jan 1;7(1):166-182. doi: 10.1039/c5sc03635f. Epub 2015 Nov 25. PMID:29896342[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Michaud G, Visini R, Bergmann M, Salerno G, Bosco R, Gillon E, Richichi B, Nativi C, Imberty A, Stocker A, Darbre T, Reymond JL. Overcoming antibiotic resistance in Pseudomonas aeruginosa biofilms using glycopeptide dendrimers. Chem Sci. 2016 Jan 1;7(1):166-182. doi: 10.1039/c5sc03635f. Epub 2015 Nov 25. PMID:29896342 doi:http://dx.doi.org/10.1039/c5sc03635f
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