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| | ==Structure of PfIMP2 (Immune Mapped Protein 2 from Plasmodium falciparum) - an antigenic protein== | | ==Structure of PfIMP2 (Immune Mapped Protein 2 from Plasmodium falciparum) - an antigenic protein== |
| - | <StructureSection load='5lg9' size='340' side='right'caption='[[5lg9]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='5lg9' size='340' side='right'caption='[[5lg9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lg9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LG9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LG9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF3D7_0730400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lg9 OCA], [http://pdbe.org/5lg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lg9 RCSB], [http://www.ebi.ac.uk/pdbsum/5lg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lg9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lg9 OCA], [https://pdbe.org/5lg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lg9 RCSB], [https://www.ebi.ac.uk/pdbsum/5lg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lg9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8IBF5_PLAF7 Q8IBF5_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Plaf7]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Benjamin, S V]] | + | [[Category: Benjamin SV]] |
| - | [[Category: Matthews, S J]] | + | [[Category: Matthews SJ]] |
| - | [[Category: Antigen]]
| + | |
| - | [[Category: Apicomplexan]]
| + | |
| - | [[Category: Immune mapped protein]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunogenic]]
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| Structural highlights
Function
Q8IBF5_PLAF7
Publication Abstract from PubMed
The immune mapped protein 1 (IMP1) was first identified as a protective antigen in Eimeria maxima and described as vaccine candidate and invasion factor in Toxoplasma gondii. We show here that TgIMP1 localizes to the inner leaflet of plasma membrane (PM) via dual acylation. Mutations either in the N-terminal myristoylation or palmitoylation sites (G2 and C5) cause relocalization of TgIMP1 to the cytosol. The first 11 amino acids are sufficient for PM targeting and the presence of lysine (K7) is critical. Disruption of TgIMP1 gene by double homologous recombination revealed no invasion defect or any measurable alteration in the lytic cycle of tachyzoites. Following immunization with TgIMP1 DNA vaccine, mice challenged with either wild type or IMP1-ko parasites showed no significant difference in protection. The sequence analysis identified a structured C-terminal domain that is present in a broader family of IMP1-like proteins conserved across the members of Apicomplexa. We present the solution structure of this domain determined from NMR data and describe a new protein fold not seen before.
Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold.,Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre D Biochim Biophys Acta. 2016 Nov 22;1865(2):208-219. doi:, 10.1016/j.bbapap.2016.11.010. PMID:27888074[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre D. Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold. Biochim Biophys Acta. 2016 Nov 22;1865(2):208-219. doi:, 10.1016/j.bbapap.2016.11.010. PMID:27888074 doi:http://dx.doi.org/10.1016/j.bbapap.2016.11.010
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