This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2bep

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2bep.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2bep.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2bep| PDB=2bep | SCENE= }}
{{STRUCTURE_2bep| PDB=2bep | SCENE= }}
-
'''CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K'''
+
===CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K===
-
==Overview==
+
<!--
-
Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15723079}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15723079 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15723079}}
==About this Structure==
==About this Structure==
Line 38: Line 42:
[[Category: Structural proteomics in europe]]
[[Category: Structural proteomics in europe]]
[[Category: Ubiquitin,e2 conjugating enzyme,protein degradation]]
[[Category: Ubiquitin,e2 conjugating enzyme,protein degradation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:17:39 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:28:17 2008''

Revision as of 03:28, 28 July 2008

Image:2bep.png

Template:STRUCTURE 2bep

CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K

Template:ABSTRACT PUBMED 15723079

About this Structure

2BEP is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079

Page seeded by OCA on Mon Jul 28 06:28:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bep"
Personal tools