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4tnl

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Revision as of 10:47, 15 March 2023

1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL

Structural highlights

4tnl is a 1 chain structure with sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THER_BACTH Extracellular zinc metalloprotease.

Publication Abstract from PubMed

The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 mus after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 mus after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 A. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.

Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.,Kern J, Tran R, Alonso-Mori R, Koroidov S, Echols N, Hattne J, Ibrahim M, Gul S, Laksmono H, Sierra RG, Gildea RJ, Han G, Hellmich J, Lassalle-Kaiser B, Chatterjee R, Brewster AS, Stan CA, Glockner C, Lampe A, DiFiore D, Milathianaki D, Fry AR, Seibert MM, Koglin JE, Gallo E, Uhlig J, Sokaras D, Weng TC, Zwart PH, Skinner DE, Bogan MJ, Messerschmidt M, Glatzel P, Williams GJ, Boutet S, Adams PD, Zouni A, Messinger J, Sauter NK, Bergmann U, Yano J, Yachandra VK Nat Commun. 2014 Jul 9;5:4371. doi: 10.1038/ncomms5371. PMID:25006873^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kern J, Tran R, Alonso-Mori R, Koroidov S, Echols N, Hattne J, Ibrahim M, Gul S, Laksmono H, Sierra RG, Gildea RJ, Han G, Hellmich J, Lassalle-Kaiser B, Chatterjee R, Brewster AS, Stan CA, Glockner C, Lampe A, DiFiore D, Milathianaki D, Fry AR, Seibert MM, Koglin JE, Gallo E, Uhlig J, Sokaras D, Weng TC, Zwart PH, Skinner DE, Bogan MJ, Messerschmidt M, Glatzel P, Williams GJ, Boutet S, Adams PD, Zouni A, Messinger J, Sauter NK, Bergmann U, Yano J, Yachandra VK. Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy. Nat Commun. 2014 Jul 9;5:4371. doi: 10.1038/ncomms5371. PMID:25006873 doi:http://dx.doi.org/10.1038/ncomms5371

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4tnl"

@@ Line 3: / Line 3: @@
 <StructureSection load='4tnl' size='340' side='right'caption='[[4tnl]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4tnl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4tnl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnl OCA], [https://pdbe.org/4tnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnl RCSB], [https://www.ebi.ac.uk/pdbsum/4tnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnl ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnl OCA], [http://pdbe.org/4tnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnl RCSB], [http://www.ebi.ac.uk/pdbsum/4tnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnl ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.
+[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH] Extracellular zinc metalloprotease.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 20: @@
 ==See Also==
-*[[Thermolysin|Thermolysin]]
+*[[Thermolysin 3D structures|Thermolysin 3D structures]]
 == References ==
 <references/>
@@ Line 28: / Line 27: @@
 [[Category: Bacillus thermoproteolyticus]]
 [[Category: Large Structures]]
-[[Category: Thermolysin]]
+[[Category: Adams PD]]
-[[Category: Adams, P D]]
+[[Category: Alonso-Mori R]]
-[[Category: Alonso-Mori, R]]
+[[Category: Bergmann U]]
-[[Category: Bergmann, U]]
+[[Category: Bogan MJ]]
-[[Category: Bogan, M J]]
+[[Category: Boutet S]]
-[[Category: Boutet, S]]
+[[Category: Brewster A]]
-[[Category: Brewster, A]]
+[[Category: Chatterjee R]]
-[[Category: Chatterjee, R]]
+[[Category: DiFiore D]]
-[[Category: DiFiore, D]]
+[[Category: Echols N]]
-[[Category: Echols, N]]
+[[Category: Fry AR]]
-[[Category: Fry, A R]]
+[[Category: Gallo E]]
-[[Category: Gallo, E]]
+[[Category: Gildea RJ]]
-[[Category: Gildea, R J]]
+[[Category: Glatzel P]]
-[[Category: Glatzel, P]]
+[[Category: Gloeckner C]]
-[[Category: Gloeckner, C]]
+[[Category: Gul S]]
-[[Category: Gul, S]]
+[[Category: Han G]]
-[[Category: Han, G]]
+[[Category: Hattne J]]
-[[Category: Hattne, J]]
+[[Category: Hellmich J]]
-[[Category: Hellmich, J]]
+[[Category: Ibrahim M]]
-[[Category: Ibrahim, M]]
+[[Category: Kern J]]
-[[Category: Kern, J]]
+[[Category: Koglin JE]]
-[[Category: Koglin, J E]]
+[[Category: Koroidov S]]
-[[Category: Koroidov, S]]
+[[Category: Laksmono H]]
-[[Category: Laksmono, H]]
+[[Category: Lampe A]]
-[[Category: Lampe, A]]
+[[Category: Lassalle-Kaiser B]]
-[[Category: Lassalle-Kaiser, B]]
+[[Category: Messerschmidt M]]
-[[Category: Messerschmidt, M]]
+[[Category: Messinger J]]
-[[Category: Messinger, J]]
+[[Category: Milathianaki D]]
-[[Category: Milathianaki, D]]
+[[Category: Sauter NK]]
-[[Category: Sauter, N K]]
+[[Category: Seibert MM]]
-[[Category: Seibert, M M]]
+[[Category: Sierra RG]]
-[[Category: Sierra, R G]]
+[[Category: Skinner DE]]
-[[Category: Skinner, D E]]
+[[Category: Sokaras D]]
-[[Category: Sokaras, D]]
+[[Category: Stan CA]]
-[[Category: Stan, C A]]
+[[Category: Tran R]]
-[[Category: Tran, R]]
+[[Category: Uhlig J]]
-[[Category: Uhlig, J]]
+[[Category: Weng T-C]]
-[[Category: Weng, T C]]
+[[Category: Williams GJ]]
-[[Category: Williams, G J]]
+[[Category: Yachandra VK]]
-[[Category: Yachandra, V K]]
+[[Category: Yano J]]
-[[Category: Yano, J]]
+[[Category: Zouni A]]
-[[Category: Zouni, A]]
+[[Category: Zwart PH]]
-[[Category: Zwart, P H]]
-[[Category: Hydrolase]]
-[[Category: X-ray free electron laser]]
-[[Category: Zn protease]]

4tnl

From Proteopedia

Revision as of 10:47, 15 March 2023

1.8 A resolution room temperature structure of Thermolysin recorded using an XFEL

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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