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4tr2

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Revision as of 10:51, 15 March 2023

Crystal structure of PvSUB1

Structural highlights

4tr2 is a 2 chain structure with sequence from Plasmodium vivax. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

E6Y8B9_PLAVI

Publication Abstract from PubMed

The Plasmodium subtilase SUB1 plays a pivotal role during the egress of malaria parasites from host hepatocytes and erythrocytes. Here we report the crystal structure of full-length SUB1 from the human-infecting parasite Plasmodium vivax, revealing a bacterial-like catalytic domain in complex with a Plasmodium-specific prodomain. The latter displays a novel architecture with an amino-terminal insertion that functions as a 'belt', embracing the catalytic domain to further stabilize the quaternary structure of the pre-protease, and undergoes calcium-dependent autoprocessing during subsequent activation. Although dispensable for recombinant enzymatic activity, the SUB1 'belt' could not be deleted in Plasmodium berghei, suggesting an essential role of this domain for parasite development in vivo. The SUB1 structure not only provides a valuable platform to develop new anti-malarial candidates against this promising drug target, but also defines the Plasmodium-specific 'belt' domain as a key calcium-dependent regulator of SUB1 during parasite egress from host cells.

A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase.,Giganti D, Bouillon A, Tawk L, Robert F, Martinez M, Crublet E, Weber P, Girard-Blanc C, Petres S, Haouz A, Hernandez JF, Mercereau-Puijalon O, Alzari PM, Barale JC Nat Commun. 2014 Sep 10;5:4833. doi: 10.1038/ncomms5833. PMID:25204226^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Giganti D, Bouillon A, Tawk L, Robert F, Martinez M, Crublet E, Weber P, Girard-Blanc C, Petres S, Haouz A, Hernandez JF, Mercereau-Puijalon O, Alzari PM, Barale JC. A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase. Nat Commun. 2014 Sep 10;5:4833. doi: 10.1038/ncomms5833. PMID:25204226 doi:http://dx.doi.org/10.1038/ncomms5833

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4tr2"

@@ Line 3: / Line 3: @@
 <StructureSection load='4tr2' size='340' side='right'caption='[[4tr2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4tr2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TR2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TR2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4tr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TR2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sub1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5855 Haemamoeba vivax])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tr2 OCA], [https://pdbe.org/4tr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tr2 RCSB], [https://www.ebi.ac.uk/pdbsum/4tr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tr2 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tr2 OCA], [http://pdbe.org/4tr2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tr2 RCSB], [http://www.ebi.ac.uk/pdbsum/4tr2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tr2 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/E6Y8B9_PLAVI E6Y8B9_PLAVI]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Haemamoeba vivax]]
 [[Category: Large Structures]]
-[[Category: Alzari, P M]]
+[[Category: Plasmodium vivax]]
-[[Category: Barale, J C]]
+[[Category: Alzari PM]]
-[[Category: Bouillon, A]]
+[[Category: Barale JC]]
-[[Category: Giganti, D]]
+[[Category: Bouillon A]]
-[[Category: Girard-Blanc, C]]
+[[Category: Giganti D]]
-[[Category: Haouz, A]]
+[[Category: Girard-Blanc C]]
-[[Category: Martinez, M]]
+[[Category: Haouz A]]
-[[Category: Petres, S]]
+[[Category: Martinez M]]
-[[Category: Weber, P]]
+[[Category: Petres S]]
-[[Category: Calcium-binding]]
+[[Category: Weber P]]
-[[Category: Hydrolase]]
-[[Category: Plasmodium egress protease]]

4tr2

From Proteopedia

Revision as of 10:51, 15 March 2023

Crystal structure of PvSUB1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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