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Laccase

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== Function ==
== Function ==
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'''Laccase''' (Lac) is a multi-copper protein which uses molecular oxygen to oxidize various aromatic and non-aromatic compounds by a radical-catalyzed reaction mechanism</ref><ref>PMID:15036303</ref>
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'''Laccase''' (Lac) is a multi-copper protein which uses molecular oxygen to oxidize various aromatic and non-aromatic compounds by a radical-catalyzed reaction mechanism</ref><ref>PMID:15036303</ref>. '''Laccase 2''' (Lac2) acts in lignin degradation and in detoxification of lignin products. Typically, laccases show a three cupredoxin-domain folding<ref>PMID:25586560</ref>. '''Two-domain laccase''' or small lactase have unusual resistance to inhibitors<ref>PMID:25778839</ref>. '''CotA laccase''' belongs to the multi-copper oxidase family.
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'''CotA laccase''' belongs to the multi-copper oxidase family.
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The multi-copper oxidases constitute a family of enzymes whose
The multi-copper oxidases constitute a family of enzymes whose
principal members are laccase (benzenediol oxygen oxidoreductase,
principal members are laccase (benzenediol oxygen oxidoreductase,
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For laccase with nitrotyrosine modification see [[Nitrotyrosine]].
For laccase with nitrotyrosine modification see [[Nitrotyrosine]].
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== Relevance ==
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Laccase from various fungi is used in adsorption of dyes from polluted environment</ref><ref>PMID:30997348</ref>. Laccases play an important role in food industry, paper and pulp industry, textile industry, synthetic chemistry, cosmetics, soil bioremediation and biodegradation of phenolic pollutants<ref>PMID:21755038</ref>.
== Structural highlights ==
== Structural highlights ==
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it to the trinuclear centre.
it to the trinuclear centre.
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==3D structures of laccase==
[[Laccase 3D structures]]
[[Laccase 3D structures]]
</StructureSection>
</StructureSection>
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==3D structures of CotA laccase==
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==3D structures of laccase==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
{{#tree:id=OrganizedByTopic|openlevels=0|
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*Laccase
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**[[1v10]] – Lac + Cu – ''Rigidoporus lignosus''<br />
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**[[2h5u]] – Lac + Cu – ''Trametes maxima''<br />
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**[[2hzh]] – Lac + Cu – ''Trametes ochracea''<br />
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**[[3x1b]] – Lac + Cu – ''Lentinus''<br />
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**[[5anh]] – Lac + Cu – ''Basidiomycete''<br />
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**[[5ehf]] – Lac + Cu – ''Antrodiella faginea''<br />
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**[[4a2d]], [[4a2e]], [[4a2f]], [[4a2g]], [[4a2h]], [[5a7e]] – Lac + Cu – ''Coriolopsis gallica''<br />
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**[[4jhu]], [[4jhv]] – Lac + Cu – ''Coriolopsis caperata''<br />
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**[[3t6v]], [[3t6w]], [[3t6x]], [[3t6z]], [[3t71]] – Lac + Cu – ''Steccherinum ochraceum''<br />
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**[[3sqr]], [[4x4k]] – BaLac + Cu – ''Botrytis aclada''<br />
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**[[3v9e]] – BaLac (mutant) + Cu <br />
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**[[2xuw]], [[2xvb]] – TtLac – ''Thermus thermophilus''<br />
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**[[2yap]], [[5jx9]], [[5k0d]], [[5k15]], [[5k3k]], [[5k5k]], [[5k7a]], [[5k84]] – TtLac + Cu <br />
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**[[2xu9]] – TtLac (mutant) + Cu <br />
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**[[2yae]], [[5jrr]] – TtLac + Cu + O2 <br />
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**[[2yaf]], [[2yah]], [[2yam]], [[2yao]], [[2yaq]], [[2yar]], [[5afa]] – TtLac + Cu + OH <br />
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**[[2xyb]] – Lac + Cu + O2 – ''Pycnoporus cinnabarinus'' <br />
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**[[3pps]] – Lac + Cu + O2 – ''Thielavia arenaria'' <br />
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**[[3cg8]] – Lac + Cu + O – ''Streptomyces coelicolor'' <br />
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**[[3tbb]] – Lac + Cu + O2 – ''Streptomyces viridosporus'' <br />
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**[[4f7k]] – Lac – uncultured bacterium<br />
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**[[5nq7]], [[5nq8]], [[5nq9]] – Lac + Cu + O2 – ''Trametes sanguinea''<br />
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**[[5z1x]], [[5z22]] – Lac + Cu + O2 – ''Cerrena''<br />
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*Laccase 1
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**[[3qpk]] – MaLac1 + Cu – ''Melanocarpus albomyces''<br />
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**[[1gw0]], [[2ih8]], [[2ih9]], [[2q9o]] – MaLac1 + Cu + O <br />
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**[[3dkh]] – MaLac1 (mutant) + Cu + O2<br />
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**[[3fu7]], [[3fu8]], [[3fu9]] – MaLac1 + Cu + O2 + phenol derivative<br />
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**[[1hfu]] – icfLac1 + Cu – inky cap fungus<br />
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**[[5ldu]] – wrfLac1 + Cu – white-rot fungus<br />
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**[[6f5k]] – Lac1 + Cu + OH – ''Myceliophthora thermophila'' <br />
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*Laccase 2
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**[[1a65]] – icfLac2 + Cu + O <br />
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**[[1gyc]], [[3fpx]], [[3kw7]], [[3pxl]] – wrfLac2 + Cu <br />
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**[[3v9c]] – wrfLac2 + Cu + O2<br />
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**[[1kya]] – Lac2 + Cu + arylamine – ''Trametes versicolor''<br />
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**[[5e9n]], [[6rgh]] – SmLac2 + Cu – ''Steccherinum murashkinskyi''<br />
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**[[5mej]], [[5mew]], [[5mhu]], [[5mhv]], [[5mhw]], [[5mhx]], [[5mhy]], [[5mhz]], [[5mi1]], [[5mi2]], [[5mia]], [[5mib]], [[5mic]], [[5mid]], [[5mie]], [[5mig]], [[6rgp]], [[6rhh]], [[6rhi]], [[6rho]], [[6rhp]], [[6rhr]], [[6rhu]], [[6rhx]], [[6ri0]], [[6ri2]], [[6ri4]], [[6ri6]], [[6ri8]], [[6rii]], [[6rik]], [[6ril]] – SmLac2 + Cu + O2<br />
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*Two-domain laccase
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**[[2zwn]] – Lac + Cu + Cu-O-Cu – metagenomes<br />
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**[[5lhl]] – SgLac + Cu + O2 – ''Streptomyces griseoflavus'' <br />
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**[[5mkm]], [[5o3k]], [[5o4i]], [[5o4q]], [[6fc7]], [[6fdj]] – SgLac (mutant) + Cu + O2 + O <br />
*CotA laccase
*CotA laccase

Revision as of 10:04, 16 May 2019

CotA laccase complex with glycerol, O2 and Cu+2 (orange), 1w6l

Drag the structure with the mouse to rotate

3D structures of laccase

Updated on 16-May-2019

See Blue copper oxidase CueO

References

  1. ↑ Claus H. Laccases: structure, reactions, distribution. Micron. 2004;35(1-2):93-6. doi: 10.1016/j.micron.2003.10.029. PMID:15036303 doi:http://dx.doi.org/10.1016/j.micron.2003.10.029
  2. ↑ Pardo I, Camarero S. Laccase engineering by rational and evolutionary design. Cell Mol Life Sci. 2015 Mar;72(5):897-910. doi: 10.1007/s00018-014-1824-8. Epub, 2015 Jan 14. PMID:25586560 doi:http://dx.doi.org/10.1007/s00018-014-1824-8
  3. ↑ Trubitsina LI, Tishchenko SV, Gabdulkhakov AG, Lisov AV, Zakharova MV, Leontievsky AA. Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes. Biochimie. 2015 May;112:151-9. doi: 10.1016/j.biochi.2015.03.005. Epub 2015 Mar, 13. PMID:25778839 doi:http://dx.doi.org/10.1016/j.biochi.2015.03.005
  4. ↑ Hullo MF, Moszer I, Danchin A, Martin-Verstraete I. CotA of Bacillus subtilis is a copper-dependent laccase. J Bacteriol. 2001 Sep;183(18):5426-30. PMID:11514528
  5. ↑ Bento I, Martins LO, Gato Lopes G, Armenia Carrondo M, Lindley PF. Dioxygen reduction by multi-copper oxidases; a structural perspective. Dalton Trans. 2005 Nov 7;(21):3507-13. Epub 2005 Sep 27. PMID:16234932 doi:10.1039/b504806k
  6. ↑ Bankole PO, Adekunle AA, Govindwar SP. Demethylation and desulfonation of textile industry dye, Thiazole Yellow G by Aspergillus niger LAG. Biotechnol Rep (Amst). 2019 Mar 28;23:e00327. doi: 10.1016/j.btre.2019.e00327., eCollection 2019 Sep. PMID:30997348 doi:http://dx.doi.org/10.1016/j.btre.2019.e00327
  7. ↑ Shraddha, Shekher R, Sehgal S, Kamthania M, Kumar A. Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme Res. 2011;2011:217861. doi: 10.4061/2011/217861. Epub 2011 Jun 21. PMID:21755038 doi:http://dx.doi.org/10.4061/2011/217861
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