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| | <StructureSection load='5z08' size='340' side='right'caption='[[5z08]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5z08' size='340' side='right'caption='[[5z08]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5z08]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Acremonium_alabamense Acremonium alabamense] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z08 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z08 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5z08]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Thermothielavioides_terrestris_NRRL_8126 Thermothielavioides terrestris NRRL 8126]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z08 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0061880 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), THITE_2065850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=578455 Acremonium alabamense]), THITE_2113209 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=578455 Acremonium alabamense])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.199Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z08 OCA], [http://pdbe.org/5z08 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z08 RCSB], [http://www.ebi.ac.uk/pdbsum/5z08 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z08 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z08 OCA], [https://pdbe.org/5z08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z08 RCSB], [https://www.ebi.ac.uk/pdbsum/5z08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z08 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/G0SFF7_CHATD G0SFF7_CHATD] |
| - | The kinetochore is a proteinaceous complex that is essential for proper chromosome segregation. As a core member of the inner kinetochore, defects of each subunit in the CENP-H/I/K complex cause dysfunction of kinetochore that leads to chromosome mis-segregation and cell death. However, how the CENP-H/I/K complex assembles and promotes kinetochore function are poorly understood. We here determined the crystal structures of CENP-I N-terminus alone from Chaetomium thermophilum and its complex with CENP-H/K from Thielavia terrestris, and verified the identified interactions. The structures and biochemical analyses show that CENP-H and CENP-K form a heterodimer through both N- and C-terminal interactions. CENP-I integrates into the CENP-H/K complex by binding to the C-terminus of CENP-H, leading to formation of the ternary complex in which CENP-H is sandwiched between CENP-K and CENP-I. Our sequence comparisons and mutational analyses showed that this architecture of the CENP-H/I/K complex is conserved in human. Mutating the binding interfaces of CENP-H for either CENP-K or CENP-I significantly reduced their localizations at centromeres and induced massive chromosome alignment defects during mitosis, suggesting that the identified interactions are critical for CENP-H/I/K complex assembly at the centromere and kinetochore function. Altogether, our findings unveil the evolutionarily conserved assembly mechanism of the CENP-H/I/K complex that is critical for proper chromosome alignment.
| + | |
| - | | + | |
| - | Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment.,Hu L, Huang H, Hei M, Yang Y, Li S, Liu Y, Dou Z, Wu M, Li J, Wang GZ, Yao X, Liu H, He X, Tian W Nucleic Acids Res. 2019 Jan 10;47(1):468-479. doi: 10.1093/nar/gky1108. PMID:30407575<ref>PMID:30407575</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5z08" style="background-color:#fffaf0;"></div>
| + | |
| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Centromere protein 3D structure|Centromere protein 3D structure]] | | *[[Centromere protein 3D structure|Centromere protein 3D structure]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acremonium alabamense]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Chatd]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: He, X]] | + | [[Category: Thermothielavioides terrestris NRRL 8126]] |
| - | [[Category: Hu, L Q]] | + | [[Category: He X]] |
| - | [[Category: Tian, W]] | + | [[Category: Hu LQ]] |
| - | [[Category: Cell cycle]] | + | [[Category: Tian W]] |
| - | [[Category: Centromere]]
| + | |
| - | [[Category: Kinetochore]]
| + | |
| - | [[Category: Protein complex]]
| + | |
