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| | <StructureSection load='5zn8' size='340' side='right'caption='[[5zn8]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='5zn8' size='340' side='right'caption='[[5zn8]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZN8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZN8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BMJ37_14840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zn8 OCA], [http://pdbe.org/5zn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zn8 RCSB], [http://www.ebi.ac.uk/pdbsum/5zn8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zn8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zn8 OCA], [https://pdbe.org/5zn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zn8 RCSB], [https://www.ebi.ac.uk/pdbsum/5zn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zn8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A223CGG0_9BACI A0A223CGG0_9BACI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, J]] | + | [[Category: Chen J]] |
| - | [[Category: Shang, F]] | + | [[Category: Shang F]] |
| - | [[Category: Wang, L]] | + | [[Category: Wang L]] |
| - | [[Category: Xu, Y]] | + | [[Category: Xu Y]] |
| - | [[Category: Bacillus subtili]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Nicotinamidase]]
| + | |
| - | [[Category: Pnca]]
| + | |
| Structural highlights
Function
A0A223CGG0_9BACI
Publication Abstract from PubMed
The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from Bacillus subtilis (BsPncA) at 2.0A resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members.
Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis.,Shang F, Chen J, Wang L, Jin L, Zou L, Bu T, Dong Y, Ha NC, Nam KH, Quan C, Xu Y Biochem Biophys Res Commun. 2018 Aug 11. pii: S0006-291X(18)31749-2. doi:, 10.1016/j.bbrc.2018.08.067. PMID:30107912[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shang F, Chen J, Wang L, Jin L, Zou L, Bu T, Dong Y, Ha NC, Nam KH, Quan C, Xu Y. Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis. Biochem Biophys Res Commun. 2018 Aug 11. pii: S0006-291X(18)31749-2. doi:, 10.1016/j.bbrc.2018.08.067. PMID:30107912 doi:http://dx.doi.org/10.1016/j.bbrc.2018.08.067
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