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| | <StructureSection load='6jqs' size='340' side='right'caption='[[6jqs]], [[Resolution|resolution]] 2.09Å' scene=''> | | <StructureSection load='6jqs' size='340' side='right'caption='[[6jqs]], [[Resolution|resolution]] 2.09Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jqs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Paenisporosarcina_sp._tg-14 Paenisporosarcina sp. tg-14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JQS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JQS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jqs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenisporosarcina_sp._TG-14 Paenisporosarcina sp. TG-14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JQS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jqs OCA], [http://pdbe.org/6jqs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jqs RCSB], [http://www.ebi.ac.uk/pdbsum/6jqs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jqs ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jqs OCA], [https://pdbe.org/6jqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jqs RCSB], [https://www.ebi.ac.uk/pdbsum/6jqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jqs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A4V8H024_9BACL A0A4V8H024_9BACL] |
| - | In cold and harsh environments such as glaciers and sediments in ice cores, microbes can survive by forming spores. Spores are composed of a thick coat protein, which protects against external factors such as heat-shock, high salinity, and nutrient deficiency. GerE is a key transcription factor involved in spore coat protein expression in the mother cell during sporulation. GerE regulates transcription during the late sporulation stage by directly binding to the promoter of cotB gene. Here, we report the crystal structure of PaGerE at 2.09A resolution from Paenisporosarcina sp. TG-14, which was isolated from the Taylor glacier. The PaGerE structure is composed of four alpha-helices and adopts a helix-turn-helix architecture with 68amino acid residues. Based on our DNA binding analysis, the PaGerE binds to the promoter region of CotB to affect protein expression. Additionally, our structural comparison studies suggest that DNA binding by PaGerE causes a conformational change in the alpha4-helix region, which may strongly induce dimerization of PaGerE.
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| - | | + | |
| - | Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14.,Lee CW, Park SH, Koh HY, Jeong CS, Hwang J, Lee SG, Youn UJ, Lee CS, Park HH, Kim HJ, Park H, Lee JH Biochem Biophys Res Commun. 2019 Apr 6. pii: S0006-291X(19)30641-2. doi:, 10.1016/j.bbrc.2019.04.019. PMID:30967265<ref>PMID:30967265</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6jqs" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Paenisporosarcina sp. tg-14]] | + | [[Category: Paenisporosarcina sp. TG-14]] |
| - | [[Category: Lee, C W]] | + | [[Category: Lee CW]] |
| - | [[Category: Lee, J H]] | + | [[Category: Lee JH]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Gere]]
| + | |
| - | [[Category: Spore]]
| + | |
