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| | <StructureSection load='4wo8' size='340' side='right'caption='[[4wo8]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='4wo8' size='340' side='right'caption='[[4wo8]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4wo8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WO8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WO8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4wo8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WO8 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Smp_194770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6183 Blood fluke])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wo8 OCA], [https://pdbe.org/4wo8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wo8 RCSB], [https://www.ebi.ac.uk/pdbsum/4wo8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wo8 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Taurocyamine_kinase Taurocyamine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.4 2.7.3.4] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wo8 OCA], [http://pdbe.org/4wo8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wo8 RCSB], [http://www.ebi.ac.uk/pdbsum/4wo8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wo8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KTRC_SCHMA KTRC_SCHMA]] This family of enzymes reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate).<ref>PMID:18765922</ref> | + | [https://www.uniprot.org/uniprot/KTRC_SCHMA KTRC_SCHMA] This family of enzymes reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate).<ref>PMID:18765922</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Blood fluke]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Taurocyamine kinase]] | + | [[Category: Schistosoma mansoni]] |
| - | [[Category: Awama, A]] | + | [[Category: Awama A]] |
| - | [[Category: Gouet, P]] | + | [[Category: Gouet P]] |
| - | [[Category: Marcillat, O]] | + | [[Category: Marcillat O]] |
| - | [[Category: Merceron, R]] | + | [[Category: Merceron R]] |
| - | [[Category: Montserret, R]] | + | [[Category: Montserret R]] |
| - | [[Category: Duplicated]]
| + | |
| - | [[Category: Substrate specificity]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Transition state]]
| + | |
| Structural highlights
Function
KTRC_SCHMA This family of enzymes reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate).[1]
Publication Abstract from PubMed
The taurocyamine kinase from the blood fluke Schistosoma mansoni (SmTK) belongs to the phosphagen kinase (PK) family and catalyzes the reversible Mg2+-dependent transfer of a phosphoryl group between ATP and taurocyamine. SmTK is derived from gene duplication, as are all known trematode TKs. Our crystallographic study of SmTK reveals the first atomic structure of both a TK and a PK with a bilobal structure. The two unliganded lobes present a canonical open conformation and interact via their respective C- and N-terminal domains at a helix-mediated interface. This spatial arrangement differs from that observed in true dimeric PKs, in which both N-terminal domains make contact. Our structures of SmTK complexed with taurocyamine or L-arginine compounds explain the mechanism by which an arginine residue of the phosphagen-specificity loop is crucial for substrate specificity. An SmTK crystal was soaked with the dead-end transition-state analog (TSA) components taurocyamine-NO32--MgADP. One SmTK monomer was observed with two bound TSAs and an asymmetric conformation, with the first lobe semi-closed and the second closed. However, isothermal titration calorimetry and enzyme kinetics experiments showed that the two lobes function independently. A small-angle X-ray scattering model of SmTK-TSA in solution with two closed active sites was generated.
The substrate -free and -bound crystal structures of the duplicated taurocyamine kinase from the human parasite Schistosoma mansoni.,Merceron R, Awama AM, Montserret R, Marcillat O, Gouet P J Biol Chem. 2015 Apr 2. pii: jbc.M114.628909. PMID:25837252[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Awama AM, Paracuellos P, Laurent S, Dissous C, Marcillat O, Gouet P. Crystallization and X-ray analysis of the Schistosoma mansoni guanidino kinase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):854-7. doi:, 10.1107/S1744309108025979. Epub 2008 Aug 20. PMID:18765922 doi:http://dx.doi.org/10.1107/S1744309108025979
- ↑ Merceron R, Awama AM, Montserret R, Marcillat O, Gouet P. The substrate -free and -bound crystal structures of the duplicated taurocyamine kinase from the human parasite Schistosoma mansoni. J Biol Chem. 2015 Apr 2. pii: jbc.M114.628909. PMID:25837252 doi:http://dx.doi.org/10.1074/jbc.M114.628909
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