4wem

From Proteopedia

(Difference between revisions)

Revision as of 07:04, 7 April 2023

Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V1

Structural highlights

4wem is a 2 chain structure with sequence from Escherichia coli and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FAEG2_ECOLX K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs.

Publication Abstract from PubMed

Enterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding.

Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.,Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H Vet Res. 2015 Feb 24;46(1):14. doi: 10.1186/s13567-015-0151-x. PMID:25828907^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H. Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies. Vet Res. 2015 Feb 24;46(1):14. doi: 10.1186/s13567-015-0151-x. PMID:25828907 doi:http://dx.doi.org/10.1186/s13567-015-0151-x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wem"

@@ Line 3: / Line 3: @@
 <StructureSection load='4wem' size='340' side='right'caption='[[4wem]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4wem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4wem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WEM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hlr|3hlr]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wem OCA], [https://pdbe.org/4wem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wem RCSB], [https://www.ebi.ac.uk/pdbsum/4wem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wem ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">faeG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wem OCA], [http://pdbe.org/4wem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wem RCSB], [http://www.ebi.ac.uk/pdbsum/4wem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wem ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/FAEG2_ECOLX FAEG2_ECOLX] K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
 *[[Antibody 3D structures|Antibody 3D structures]]
-*[[Pilin|Pilin]]
+*[[Pilin 3D structures|Pilin 3D structures]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Camelus glama]]
+[[Category: Lama glama]]
 [[Category: Large Structures]]
-[[Category: Broeck, I Van den]]
+[[Category: De Greve H]]
-[[Category: Greve, H De]]
+[[Category: De Kerpel M]]
-[[Category: Kerpel, M De]]
+[[Category: Moonens K]]
-[[Category: Moonens, K]]
+[[Category: Pardon E]]
-[[Category: Pardon, E]]
+[[Category: Remaut H]]
-[[Category: Remaut, H]]
+[[Category: Van den Broeck I]]
-[[Category: Adhesin]]
-[[Category: Complex]]
-[[Category: Llama single domain antibody]]
-[[Category: Nanobody]]
-[[Category: Structural protein]]

4wem

From Proteopedia

Revision as of 07:04, 7 April 2023

Co-complex structure of the F4 fimbrial adhesin FaeG variant ac with llama single domain antibody V1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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