This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4ux5

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:48, 29 March 2023

Structure of DNA complex of PCG2

Structural highlights

4ux5 is a 4 chain structure with sequence from Candida albicans and Pyricularia oryzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

L7I1M8_MAGOY

Publication Abstract from PubMed

The MBP1 family proteins are the DNA binding subunits of MBF cell-cycle transcription factor complexes and contain an N terminal winged helix-turn-helix (wHTH) DNA binding domain (DBD). Although the DNA binding mechanism of MBP1 from Saccharomyces cerevisiae has been extensively studied, the structural framework and the DNA binding mode of other MBP1 family proteins remains to be disclosed. Here, we determined the crystal structure of the DBD of PCG2, the Magnaporthe oryzae orthologue of MBP1, bound to MCB-DNA. The structure revealed that the wing, the 20-loop, helix A and helix B in PCG2-DBD are important elements for DNA binding. Unlike previously characterized wHTH proteins, PCG2-DBD utilizes the wing and helix-B to bind the minor groove and the major groove of the MCB-DNA whilst the 20-loop and helix A interact non-specifically with DNA. Notably, two glutamines Q89 and Q82 within the wing were found to recognize the MCB core CGCG sequence through making hydrogen bond interactions. Further in vitro assays confirmed essential roles of Q89 and Q82 in the DNA binding. These data together indicate that the MBP1 homologue PCG2 employs an unusual mode of binding to target DNA and demonstrate the versatility of wHTH domains.

Structural basis of DNA recognition by PCG2 reveals a novel DNA binding mode for winged helix-turn-helix domains.,Liu J, Huang J, Zhao Y, Liu H, Wang D, Yang J, Zhao W, Taylor IA, Peng YL Nucleic Acids Res. 2014 Dec 29. pii: gku1351. PMID:25550425^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu J, Huang J, Zhao Y, Liu H, Wang D, Yang J, Zhao W, Taylor IA, Peng YL. Structural basis of DNA recognition by PCG2 reveals a novel DNA binding mode for winged helix-turn-helix domains. Nucleic Acids Res. 2014 Dec 29. pii: gku1351. PMID:25550425 doi:http://dx.doi.org/10.1093/nar/gku1351

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ux5"

@@ Line 3: / Line 3: @@
 <StructureSection load='4ux5' size='340' side='right'caption='[[4ux5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ux5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Magnaporthe_oryzae Magnaporthe oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UX5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ux5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans] and [https://en.wikipedia.org/wiki/Pyricularia_oryzae Pyricularia oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UX5 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ux5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux5 OCA], [http://pdbe.org/4ux5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ux5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ux5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ux5 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ux5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ux5 OCA], [https://pdbe.org/4ux5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ux5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ux5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ux5 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/L7I1M8_MAGOY L7I1M8_MAGOY]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Candida albicans]]
 [[Category: Large Structures]]
-[[Category: Magnaporthe oryzae]]
+[[Category: Pyricularia oryzae]]
-[[Category: Huang, J]]
+[[Category: Huang J]]
-[[Category: Liu, H]]
+[[Category: Liu H]]
-[[Category: Liu, J]]
+[[Category: Liu J]]
-[[Category: Peng, Y]]
+[[Category: Peng Y]]
-[[Category: Taylor, I A]]
+[[Category: Taylor IA]]
-[[Category: Wang, D]]
+[[Category: Wang D]]
-[[Category: Yang, J]]
+[[Category: Yang J]]
-[[Category: Zhao, W]]
+[[Category: Zhao W]]
-[[Category: Zhao, Y]]
+[[Category: Zhao Y]]
-[[Category: Transcription]]
-[[Category: Transcription factor]]
-[[Category: Virulence gene]]

4ux5

From Proteopedia

Revision as of 07:48, 29 March 2023

Structure of DNA complex of PCG2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox