This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2j18
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2j18. | + | [[Image:2j18.jpg|left|200px]]<br /><applet load="2j18" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j18" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2j18, resolution 1.75Å" /> | caption="2j18, resolution 1.75Å" /> | ||
'''CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)'''<br /> | '''CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2J18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, BR and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] | + | 2J18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, BR and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Br Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J18 OCA]. |
==Reference== | ==Reference== | ||
| Line 35: | Line 34: | ||
[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:43:58 2007'' |
Revision as of 17:34, 18 December 2007
|
CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)
Overview
The X-ray crystallographic analysis of redox-active systems may be, complicated by photoreduction. Although radiolytic reduction by the, probing X-ray beam may be exploited to generate otherwise short-lived, reaction intermediates of metalloproteins, it is generally an undesired, feature. Here, the X-ray-induced reduction of the three heme proteins, myoglobin, cytochrome P450cam and chloroperoxidase has been followed by, on-line UV-Vis absorption spectroscopy. All three systems showed a very, rapid reduction of the heme iron. In chloroperoxidase the change of the, ionization state from ferric to ferrous heme is associated with a movement, of the heme-coordinating water molecule. The influence of the energy of, the incident X-ray photons and of the presence of scavengers on the, apparent reduction rate of ferric myoglobin crystals was analyzed.
About this Structure
2J18 is a Single protein structure of sequence from Leptoxyphium fumago with NAG, MAN, MN, BR and HEM as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:17211068
Page seeded by OCA on Tue Dec 18 19:43:58 2007
Categories: Chloride peroxidase | Leptoxyphium fumago | Single protein | Beitlich, T. | Kuhnel, K. | Schlichting, I. | Schulze-Briese, C. | Shoeman, R.L. | BR | HEM | MAN | MN | NAG | Chloride | Glycoprotein | Heme | Iron | Manganese | Metal-binding | Oxidoreductase | Peroxidase | Pyrrolidone carboxylic acid
