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4wja

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Revision as of 07:11, 7 April 2023

Crystal Structure of PAXX

Structural highlights

4wja is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PAXX_HUMAN Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ); participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:27705800). May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:25574025, PubMed:25670504, PubMed:25941166). Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (PubMed:27703001). Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed:30250067, PubMed:25670504). Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27705800). Able to restrict infection by herpesvirus 1 (HSV-1) via an unknown mechanism (PubMed:29144403).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Publication Abstract from PubMed

Non-homologous end joining (NHEJ) is a major pathway to repair DNA double-strand breaks (DSBs), which can display different types of broken ends. However, it is unclear how NHEJ factors organize to repair diverse types of DNA breaks. Here, through systematic analysis of the human NHEJ factor interactome, we identify PAXX as a direct interactor of Ku. The crystal structure of PAXX is similar to those of XRCC4 and XLF. Importantly, PAXX-deficient cells are sensitive to DSB-causing agents. Moreover, epistasis analysis demonstrates that PAXX functions together with XLF in response to ionizing radiation-induced complex DSBs, whereas they function redundantly in response to Topo2 inhibitor-induced simple DSBs. Consistently, PAXX and XLF coordinately promote the ligation of complex but not simple DNA ends in vitro. Altogether, our data identify PAXX as a new NHEJ factor and provide insight regarding the organization of NHEJ factors responding to diverse types of DSB ends.

Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.,Xing M, Yang M, Huo W, Feng F, Wei L, Jiang W, Ning S, Yan Z, Li W, Wang Q, Hou M, Dong C, Guo R, Gao G, Ji J, Zha S, Lan L, Liang H, Xu D Nat Commun. 2015 Feb 11;6:6233. doi: 10.1038/ncomms7233. PMID:25670504^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ochi T, Blackford AN, Coates J, Jhujh S, Mehmood S, Tamura N, Travers J, Wu Q, Draviam VM, Robinson CV, Blundell TL, Jackson SP. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015 Jan 9;347(6218):185-188. doi: 10.1126/science.1261971. PMID:25574025 doi:http://dx.doi.org/10.1126/science.1261971
↑ Xing M, Yang M, Huo W, Feng F, Wei L, Jiang W, Ning S, Yan Z, Li W, Wang Q, Hou M, Dong C, Guo R, Gao G, Ji J, Zha S, Lan L, Liang H, Xu D. Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 2015 Feb 11;6:6233. doi: 10.1038/ncomms7233. PMID:25670504 doi:http://dx.doi.org/10.1038/ncomms7233
↑ Craxton A, Somers J, Munnur D, Jukes-Jones R, Cain K, Malewicz M. XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair. Cell Death Differ. 2015 Jun;22(6):890-7. PMID:25941166 doi:10.1038/cdd.2015.22
↑ Chang HHY, Watanabe G, Gerodimos CA, Ochi T, Blundell TL, Jackson SP, Lieber MR. Different DNA End Configurations Dictate Which NHEJ Components Are Most Important for Joining Efficiency. J Biol Chem. 2016 Nov 18;291(47):24377-24389. PMID:27703001 doi:10.1074/jbc.M116.752329
↑ Tadi SK, Tellier-Lebègue C, Nemoz C, Drevet P, Audebert S, Roy S, Meek K, Charbonnier JB, Modesti M. PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF. Cell Rep. 2016 Oct 4;17(2):541-555. PMID:27705800 doi:10.1016/j.celrep.2016.09.026
↑ Trigg BJ, Lauer KB, Fernandes Dos Santos P, Coleman H, Balmus G, Mansur DS, Ferguson BJ. The Non-Homologous End Joining Protein PAXX Acts to Restrict HSV-1 Infection. Viruses. 2017 Nov 16;9(11):342. PMID:29144403 doi:10.3390/v9110342
↑ Craxton A, Munnur D, Jukes-Jones R, Skalka G, Langlais C, Cain K, Malewicz M. PAXX and its paralogs synergistically direct DNA polymerase λ activity in DNA repair. Nat Commun. 2018 Sep 24;9(1):3877. PMID:30250067 doi:10.1038/s41467-018-06127-y
↑ Xing M, Yang M, Huo W, Feng F, Wei L, Jiang W, Ning S, Yan Z, Li W, Wang Q, Hou M, Dong C, Guo R, Gao G, Ji J, Zha S, Lan L, Liang H, Xu D. Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 2015 Feb 11;6:6233. doi: 10.1038/ncomms7233. PMID:25670504 doi:http://dx.doi.org/10.1038/ncomms7233

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wja"

@@ Line 3: / Line 3: @@
 <StructureSection load='4wja' size='340' side='right'caption='[[4wja]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4wja]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WJA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WJA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4wja]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WJA FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C9orf142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wja OCA], [https://pdbe.org/4wja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wja RCSB], [https://www.ebi.ac.uk/pdbsum/4wja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wja ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wja OCA], [http://pdbe.org/4wja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wja RCSB], [http://www.ebi.ac.uk/pdbsum/4wja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wja ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PAXX_HUMAN PAXX_HUMAN] Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ); participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:27705800). May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:25574025, PubMed:25670504, PubMed:25941166). Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (PubMed:27703001). Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed:30250067, PubMed:25670504). Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27705800). Able to restrict infection by herpesvirus 1 (HSV-1) via an unknown mechanism (PubMed:29144403).<ref>PMID:25574025</ref> <ref>PMID:25670504</ref> <ref>PMID:25941166</ref> <ref>PMID:27703001</ref> <ref>PMID:27705800</ref> <ref>PMID:29144403</ref> <ref>PMID:30250067</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Dong, C]]
+[[Category: Dong C]]
-[[Category: Feng, F]]
+[[Category: Feng F]]
-[[Category: Gao, G]]
+[[Category: Gao G]]
-[[Category: Guo, R]]
+[[Category: Guo R]]
-[[Category: Hou, M]]
+[[Category: Hou M]]
-[[Category: Huo, W]]
+[[Category: Huo W]]
-[[Category: Ji, J]]
+[[Category: Ji J]]
-[[Category: Lan, L]]
+[[Category: Lan L]]
-[[Category: Li, W]]
+[[Category: Li W]]
-[[Category: Liang, H]]
+[[Category: Liang H]]
-[[Category: Ning, S]]
+[[Category: Ning S]]
-[[Category: Wang, Q]]
+[[Category: Wang Q]]
-[[Category: Wei, L]]
+[[Category: Wei L]]
-[[Category: Xing, M]]
+[[Category: Xing M]]
-[[Category: Xu, D]]
+[[Category: Xu D]]
-[[Category: Yan, Z]]
+[[Category: Yan Z]]
-[[Category: Yang, M]]
+[[Category: Yang M]]
-[[Category: Dna binding protein]]
-[[Category: Dna repair]]
-[[Category: Dsb]]

4wja

From Proteopedia

Revision as of 07:11, 7 April 2023

Crystal Structure of PAXX

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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