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4uzz

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Revision as of 07:54, 29 March 2023

Crystal structure of the TtIFT52-46 complex

Structural highlights

4uzz is a 2 chain structure with sequence from Tetrahymena thermophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q23KH7_TETTS

Publication Abstract from PubMed

Cilia are microtubule-based organelles that assemble via intraflagellar transport (IFT) and function as signaling hubs on eukaryotic cells. IFT relies on molecular motors and IFT complexes that mediate the contacts with ciliary cargo. To elucidate the architecture of the IFT-B complex, we reconstituted and purified the nonameric IFT-B core from Chlamydomonas reinhardtii and determined the crystal structures of C. reinhardtii IFT70/52 and Tetrahymena IFT52/46 subcomplexes. The 2.5-A resolution IFT70/52 structure shows that IFT52330-370 is buried deeply within the IFT70 tetratricopeptide repeat superhelix. Furthermore, the polycystic kidney disease protein IFT88 binds IFT52281-329 in a complex that interacts directly with IFT70/IFT52330-381 in trans. The structure of IFT52C/IFT46C was solved at 2.3 A resolution, and we show that it is essential for IFT-B core integrity by mediating interaction between IFT88/70/52/46 and IFT81/74/27/25/22 subcomplexes. Consistent with this, overexpression of mammalian IFT52C in MDCK cells is dominant-negative and causes IFT protein mislocalization and disrupted ciliogenesis. These data further rationalize several ciliogenesis phenotypes of IFT mutant strains.

Crystal structures of IFT70/52 and IFT52/46 provide insight into intraflagellar transport B core complex assembly.,Taschner M, Kotsis F, Braeuer P, Kuehn EW, Lorentzen E J Cell Biol. 2014 Oct 27;207(2):269-82. doi: 10.1083/jcb.201408002. PMID:25349261^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Taschner M, Kotsis F, Braeuer P, Kuehn EW, Lorentzen E. Crystal structures of IFT70/52 and IFT52/46 provide insight into intraflagellar transport B core complex assembly. J Cell Biol. 2014 Oct 27;207(2):269-82. doi: 10.1083/jcb.201408002. PMID:25349261 doi:http://dx.doi.org/10.1083/jcb.201408002

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4uzz"

Categories: Large Structures | Tetrahymena thermophila | Braeuer P | Lorentzen E | Taschner M

@@ Line 3: / Line 3: @@
 <StructureSection load='4uzz' size='340' side='right'caption='[[4uzz]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4uzz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetth Tetth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UZZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4uzz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UZZ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzz OCA], [http://pdbe.org/4uzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uzz RCSB], [http://www.ebi.ac.uk/pdbsum/4uzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzz ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzz OCA], [https://pdbe.org/4uzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uzz RCSB], [https://www.ebi.ac.uk/pdbsum/4uzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzz ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q23KH7_TETTS Q23KH7_TETTS]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Tetth]]
+[[Category: Tetrahymena thermophila]]
-[[Category: Braeuer, P]]
+[[Category: Braeuer P]]
-[[Category: Lorentzen, E]]
+[[Category: Lorentzen E]]
-[[Category: Taschner, M]]
+[[Category: Taschner M]]
-[[Category: Cilium]]
-[[Category: Flagellum]]
-[[Category: Ift]]
-[[Category: Intracellular transport]]
-[[Category: Motor protein]]

4uzz

From Proteopedia

Revision as of 07:54, 29 March 2023

Crystal structure of the TtIFT52-46 complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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