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4wzr

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Revision as of 21:05, 12 April 2023

Crystal structure of human Puf-A

Structural highlights

4wzr is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PUM3_HUMAN Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress (PubMed:21266351). Binds to double-stranded RNA or DNA without sequence specificity (PubMed:25512524). Involved in development of the eye and of primordial germ cells (By similarity).[UniProtKB:X1WGX5]^[1] ^[2]

Publication Abstract from PubMed

Pumilio/feminization of XX and XO animals (fem)-3 mRNA-binding factor (PUF) proteins bind sequence specifically to mRNA targets using a single-stranded RNA-binding domain comprising eight Pumilio (PUM) repeats. PUM repeats have now been identified in proteins that function in pre-rRNA processing, including human Puf-A and yeast Puf6. This is a role not previously ascribed to PUF proteins. Here we present crystal structures of human Puf-A that reveal a class of nucleic acid-binding proteins with 11 PUM repeats arranged in an "L"-like shape. In contrast to classical PUF proteins, Puf-A forms sequence-independent interactions with DNA or RNA, mediated by conserved basic residues. We demonstrate that equivalent basic residues in yeast Puf6 are important for RNA binding, pre-rRNA processing, and mRNA localization. Thus, PUM repeats can be assembled into alternative folds that bind to structured nucleic acids in addition to forming canonical eight-repeat crescent-shaped RNA-binding domains found in classical PUF proteins.

A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.,Qiu C, McCann KL, Wine RN, Baserga SJ, Hall TM Proc Natl Acad Sci U S A. 2014 Dec 15. pii: 201407634. PMID:25512524^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS. hPuf-A/KIAA0020 modulates PARP-1 cleavage upon genotoxic stress. Cancer Res. 2011 Feb 1;71(3):1126-34. PMID:21266351 doi:10.1158/0008-5472.CAN-10-1831
↑ Qiu C, McCann KL, Wine RN, Baserga SJ, Hall TM. A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization. Proc Natl Acad Sci U S A. 2014 Dec 15. pii: 201407634. PMID:25512524 doi:http://dx.doi.org/10.1073/pnas.1407634112
↑ Qiu C, McCann KL, Wine RN, Baserga SJ, Hall TM. A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization. Proc Natl Acad Sci U S A. 2014 Dec 15. pii: 201407634. PMID:25512524 doi:http://dx.doi.org/10.1073/pnas.1407634112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wzr"

Categories: Homo sapiens | Large Structures | Hall TMT | Qiu C

@@ Line 3: / Line 3: @@
 <StructureSection load='4wzr' size='340' side='right'caption='[[4wzr]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4wzr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WZR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4wzr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WZR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIAA0020, XTP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wzr OCA], [https://pdbe.org/4wzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wzr RCSB], [https://www.ebi.ac.uk/pdbsum/4wzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wzr ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wzr OCA], [http://pdbe.org/4wzr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wzr RCSB], [http://www.ebi.ac.uk/pdbsum/4wzr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wzr ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PUM3_HUMAN PUM3_HUMAN] Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress (PubMed:21266351). Binds to double-stranded RNA or DNA without sequence specificity (PubMed:25512524). Involved in development of the eye and of primordial germ cells (By similarity).[UniProtKB:X1WGX5]<ref>PMID:21266351</ref> <ref>PMID:25512524</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Hall, T M.T]]
+[[Category: Hall TMT]]
-[[Category: Qiu, C]]
+[[Category: Qiu C]]
-[[Category: Pumilio repeat protein]]
-[[Category: Rna binding protein]]

4wzr

From Proteopedia

Revision as of 21:05, 12 April 2023

Crystal structure of human Puf-A

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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