6ibp

From Proteopedia

(Difference between revisions)

Current revision

Structure of a psychrophilic CCA-adding enzyme at room temperature in ChipX microfluidic device

Structural highlights

6ibp is a 1 chain structure with sequence from Planococcus halocryophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.536Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A1C7DQ98_9BACL Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.[SAAS:SAAS00711016]

Publication Abstract from PubMed

Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.,de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Morl M, Roussel A, Girard E, Mueller-Dieckmann C, Fox GC, Olieric V, Gavira JA, Lorber B, Sauter C IUCrJ. 2019 Apr 19;6(Pt 3):454-464. doi: 10.1107/S2052252519003622. eCollection, 2019 May 1. PMID:31098026^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Morl M, Roussel A, Girard E, Mueller-Dieckmann C, Fox GC, Olieric V, Gavira JA, Lorber B, Sauter C. A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography. IUCrJ. 2019 Apr 19;6(Pt 3):454-464. doi: 10.1107/S2052252519003622. eCollection, 2019 May 1. PMID:31098026 doi:http://dx.doi.org/10.1107/S2052252519003622

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ibp"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ibp' size='340' side='right'caption='[[6ibp]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ibp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IBP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ibp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Planococcus_halocryophilus Planococcus halocryophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IBP FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/CCA_tRNA_nucleotidyltransferase CCA tRNA nucleotidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.72 2.7.7.72] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.536&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ibp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibp OCA], [http://pdbe.org/6ibp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ibp RCSB], [http://www.ebi.ac.uk/pdbsum/6ibp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibp ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ibp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibp OCA], [https://pdbe.org/6ibp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ibp RCSB], [https://www.ebi.ac.uk/pdbsum/6ibp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibp ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/A0A1C7DQ98_9BACL A0A1C7DQ98_9BACL]] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.[SAAS:SAAS00711016]
+[https://www.uniprot.org/uniprot/A0A1C7DQ98_9BACL A0A1C7DQ98_9BACL] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.[SAAS:SAAS00711016]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 6ibp" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[CCA-adding enzyme 3D structures|CCA-adding enzyme 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: CCA tRNA nucleotidyltransferase]]
 [[Category: Large Structures]]
-[[Category: Betat, H]]
+[[Category: Planococcus halocryophilus]]
-[[Category: Bluhm, A]]
+[[Category: Betat H]]
-[[Category: Hennig, O]]
+[[Category: Bluhm A]]
-[[Category: Lorber, B]]
+[[Category: Hennig O]]
-[[Category: Moerl, M]]
+[[Category: Lorber B]]
-[[Category: Rollet, K]]
+[[Category: Moerl M]]
-[[Category: Sauter, C]]
+[[Category: Rollet K]]
-[[Category: Wijn, R de]]
+[[Category: Sauter C]]
-[[Category: Chipx]]
+[[Category: De Wijn R]]
-[[Category: Psychrophilic enzyme]]
-[[Category: Rna binding protein]]
-[[Category: Trna maturation]]
-[[Category: Trna nucleotidyltransferase]]

6ibp

From Proteopedia

Current revision

Structure of a psychrophilic CCA-adding enzyme at room temperature in ChipX microfluidic device

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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