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| | <StructureSection load='6n8r' size='340' side='right'caption='[[6n8r]], [[Resolution|resolution]] 1.91Å' scene=''> | | <StructureSection load='6n8r' size='340' side='right'caption='[[6n8r]], [[Resolution|resolution]] 1.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6n8r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N8R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N8R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6n8r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N8R FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LLGL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n8r OCA], [http://pdbe.org/6n8r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n8r RCSB], [http://www.ebi.ac.uk/pdbsum/6n8r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n8r ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n8r OCA], [https://pdbe.org/6n8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n8r RCSB], [https://www.ebi.ac.uk/pdbsum/6n8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n8r ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/L2GL2_HUMAN L2GL2_HUMAN]] Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity.<ref>PMID:15632202</ref> | + | [https://www.uniprot.org/uniprot/L2GL2_HUMAN L2GL2_HUMAN] Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity.<ref>PMID:15632202</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Metazoan cell polarity is controlled by a set of highly conserved proteins. Lethal giant larvae (Lgl) functions in apical-basal polarity through phosphorylation-dependent interactions with several other proteins as well as the plasma membrane. Phosphorylation of Lgl by atypical protein kinase C (aPKC), a component of the partitioning-defective (Par) complex in epithelial cells, excludes Lgl from the apical membrane, a crucial step in the establishment of epithelial cell polarity. We present the crystal structures of human Lgl2 in both its unphosphorylated and aPKC-phosphorylated states. Lgl2 adopts a double beta-propeller structure that is unchanged by aPKC phosphorylation of an unstructured loop in its second beta-propeller, ruling out models of phosphorylation-dependent conformational change. We demonstrate that phosphorylation controls the direct binding of purified Lgl2 to negative phospholipids in vitro. We also show that a coil-helix transition of this region that is promoted by phosphatidylinositol 4,5-bisphosphate (PIP2) is also phosphorylation-dependent, implying a highly effective phosphorylative switch for membrane association.
| + | |
| - | | + | |
| - | Structural insights into the aPKC regulatory switch mechanism of the human cell polarity protein lethal giant larvae 2.,Almagor L, Ufimtsev IS, Ayer A, Li J, Weis WI Proc Natl Acad Sci U S A. 2019 May 14. pii: 1821514116. doi:, 10.1073/pnas.1821514116. PMID:31088962<ref>PMID:31088962</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6n8r" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Almagor, L]] | + | [[Category: Almagor L]] |
| - | [[Category: Weis, W I]] | + | [[Category: Weis WI]] |
| - | [[Category: Beta propeller]]
| + | |
| - | [[Category: Lgl]]
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| - | [[Category: Lipid binding protein]]
| + | |
| - | [[Category: Polarity]]
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