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4xrt

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Revision as of 17:49, 26 April 2023

Crystal structure of the di-domain ARO/CYC StfQ from the steffimycin biosynthetic pathway

Structural highlights

4xrt is a 2 chain structure with sequence from Streptomyces steffisburgensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q2PA00_9ACTN

Publication Abstract from PubMed

Aromatic polyketides make up a large class of natural products with diverse bioactivity. During biosynthesis, linear poly-beta-ketone intermediates are regiospecifically cyclized, yielding molecules with defined cyclization patterns that are crucial for polyketide bioactivity. The aromatase/cyclases (ARO/CYCs) are responsible for regiospecific cyclization of bacterial polyketides. The two most common cyclization patterns are C7-C12 and C9-C14 cyclizations. We have previously characterized three monodomain ARO/CYCs: ZhuI, TcmN, and WhiE. The last remaining uncharacterized class of ARO/CYCs is the di-domain ARO/CYCs, which catalyze C7-C12 cyclization and/or aromatization. Di-domain ARO/CYCs can further be separated into two subclasses: "nonreducing" ARO/CYCs, which act on nonreduced poly-beta-ketones, and "reducing" ARO/CYCs, which act on cyclized C9 reduced poly-beta-ketones. For years, the functional role of each domain in cyclization and aromatization for di-domain ARO/CYCs has remained a mystery. Here we present what is to our knowledge the first structural and functional analysis, along with an in-depth comparison, of the nonreducing (StfQ) and reducing (BexL) di-domain ARO/CYCs. This work completes the structural and functional characterization of mono- and di-domain ARO/CYCs in bacterial type II polyketide synthases and lays the groundwork for engineered biosynthesis of new bioactive polyketides.

Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases.,Caldara-Festin G, Jackson DR, Barajas JF, Valentic TR, Patel AB, Aguilar S, Nguyen M, Vo M, Khanna A, Sasaki E, Liu HW, Tsai SC Proc Natl Acad Sci U S A. 2015 Dec 2. pii: 201512976. PMID:26631750^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Caldara-Festin G, Jackson DR, Barajas JF, Valentic TR, Patel AB, Aguilar S, Nguyen M, Vo M, Khanna A, Sasaki E, Liu HW, Tsai SC. Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases. Proc Natl Acad Sci U S A. 2015 Dec 2. pii: 201512976. PMID:26631750 doi:http://dx.doi.org/10.1073/pnas.1512976112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xrt"

@@ Line 3: / Line 3: @@
 <StructureSection load='4xrt' size='340' side='right'caption='[[4xrt]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4xrt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_steffisburgensis"_dietz "streptomyces steffisburgensis" dietz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4xrt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_steffisburgensis Streptomyces steffisburgensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XRT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tfz|3tfz]], [[2rer|2rer]], [[2rez|2rez]], [[2kf2|2kf2]], [[4xrw|4xrw]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xrt OCA], [https://pdbe.org/4xrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xrt RCSB], [https://www.ebi.ac.uk/pdbsum/4xrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xrt ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">stfQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68271 "Streptomyces steffisburgensis" Dietz])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xrt OCA], [http://pdbe.org/4xrt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xrt RCSB], [http://www.ebi.ac.uk/pdbsum/4xrt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xrt ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q2PA00_9ACTN Q2PA00_9ACTN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Streptomyces steffisburgensis dietz]]
 [[Category: Large Structures]]
-[[Category: Aguilar, S]]
+[[Category: Streptomyces steffisburgensis]]
-[[Category: Barajas, J F]]
+[[Category: Aguilar S]]
-[[Category: Caldara-Festin, G M]]
+[[Category: Barajas JF]]
-[[Category: Jackson, D R]]
+[[Category: Caldara-Festin GM]]
-[[Category: Khanna, A]]
+[[Category: Jackson DR]]
-[[Category: Liu, H W]]
+[[Category: Khanna A]]
-[[Category: Nguyen, M]]
+[[Category: Liu H-W]]
-[[Category: Patel, A]]
+[[Category: Nguyen M]]
-[[Category: Sasaki, E]]
+[[Category: Patel A]]
-[[Category: Tsai, S C]]
+[[Category: Sasaki E]]
-[[Category: Valentic, T R]]
+[[Category: Tsai SC]]
-[[Category: Vo, M]]
+[[Category: Valentic TR]]
-[[Category: Aro/cyc]]
+[[Category: Vo M]]
-[[Category: Aromatase/cyclase]]
-[[Category: Dehydratase]]
-[[Category: Helix-grip fold]]
-[[Category: Lyase]]
-[[Category: Natural product]]
-[[Category: Polyketide]]
-[[Category: Polyketide synthase]]

4xrt

From Proteopedia

Revision as of 17:49, 26 April 2023

Crystal structure of the di-domain ARO/CYC StfQ from the steffimycin biosynthetic pathway

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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