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| | <StructureSection load='4y1g' size='340' side='right'caption='[[4y1g]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='4y1g' size='340' side='right'caption='[[4y1g]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4y1g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Y1G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4y1g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y1G FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4y0n|4y0n]], [[4y1e|4y1e]], [[4y1f|4y1f]], [[4y1r|4y1r]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1g OCA], [https://pdbe.org/4y1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y1g RCSB], [https://www.ebi.ac.uk/pdbsum/4y1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1g ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAV1875 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4y1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y1g OCA], [http://pdbe.org/4y1g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4y1g RCSB], [http://www.ebi.ac.uk/pdbsum/4y1g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4y1g ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Y1875_STAAM Y1875_STAAM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Staam]] | + | [[Category: Staphylococcus aureus subsp. aureus Mu50]] |
| - | [[Category: Kim, H J]] | + | [[Category: Kim HJ]] |
| - | [[Category: Kwon, A R]] | + | [[Category: Kwon AR]] |
| - | [[Category: Lee, B J]] | + | [[Category: Lee BJ]] |
| - | [[Category: Dj-1/pfpi/thij family]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
Y1875_STAAM
Publication Abstract from PubMed
The DJ-1/ThiJ/PfpI superfamily is a group of proteins found in diverse organisms. This superfamily includes versatile proteins, such as proteases, chaperones, heat-shock proteins and human Parkinson's disease protein. Most members of the DJ-1/ThiJ/PfpI superfamily are oligomers and are classified into subfamilies depending on discriminating quaternary structures (DJ-1, YhbO and Hsp types). SAV1875, a conserved protein from Staphylococcus aureus, is a member of the YhbO-type subfamily. However, its structure and function remain unknown. Thus, to understand the function and activity mechanism of this protein, the crystal structure of SAV1875 from S. aureus was determined. The overall fold of SAV1875 is similar to that observed for the DJ-1/ThiJ/PfpI superfamily. The cysteine residue located in the dimeric interface (Cys(105)) forms a catalytic triad with His(106) and Asp(77), and it is spontaneously oxidized to Cys(105)-SO2H in the crystal structure. To study the oxidative propensity of Cys(105) and the corresponding functional differences with changes in cysteine oxidation state, the crystal structures of SAV1875 variants E17N, E17D and C105D, and over-oxidized SAV1875 were determined. We identified SAV1875 as a novel member of the YhbO-type subfamily exhibiting chaperone function. However, if SAV1875 is over-oxidized further with H2O2, its chaperone activity is eliminated. On the basis of our study, we suggest that SAV1875 functions as a chaperone and the redox state of Cys(105) may play an important role.
Structural and functional insight into the different oxidation states of SAV1875 from Staphylococcus aureus.,Kim HJ, Kwon AR, Lee BJ Biochem J. 2016 Jan 1;473(1):55-66. doi: 10.1042/BJ20150256. Epub 2015 Oct 20. PMID:26487697[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim HJ, Kwon AR, Lee BJ. Structural and functional insight into the different oxidation states of SAV1875 from Staphylococcus aureus. Biochem J. 2016 Jan 1;473(1):55-66. doi: 10.1042/BJ20150256. Epub 2015 Oct 20. PMID:26487697 doi:http://dx.doi.org/10.1042/BJ20150256
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