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6a80

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Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with cystine

Structural highlights

6a80 is a 2 chain structure with sequence from Candidatus Liberibacter asiaticus str. psy62. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.56Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C6XGT2_LIBAP

Publication Abstract from PubMed

The amino acid-binding receptors, a component of ABC transporters, have evolved to cater to different specificities and functions. Of particular interest are cystine-binding receptors, which have shown broad specificity. In the present study, a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus (CLasTcyA) was characterized. Analysis of the CLasTcyA sequence and crystal structures in the ligand-bound state revealed novel features of CLasTcyA in comparison to related proteins. One of the unique features found in CLasTcyA structure was the positioning of the C-terminal extended loop of one chain very close to the substrate-binding site of the adjacent monomer in the asymmetric unit. The presence of a disulphide bond, unique to Candidatus Liberibacter family, holds the C-terminal extended loop in position. Analysis of the substrate-binding pocket of CLasTcyA suggested a broad specificity and a completely different orientation of the bound substrates in comparison to related protein structures. The open conformation for one of the two chains of the asymmetric unit in the Arg-bound structure revealed a limited open state (18.4 degrees ) for CLasTcyA as compared to open state of other related proteins (~ 60 degrees ). The strong interaction between Asp126 on helix-alpha5 of small domain and Arg82 (bigger domain) restricts the degree of opening in ligand-free open state. The dissociation constant of 1.26 mum by SPR and 3.7 mum by MST exhibited low affinity for the cystine. This is the first structural characterization of an l-cystine ABC transporter from plant pathogen and our results suggest that CLasTcyA may have evolved to cater to its specific needs for its survival in the host.

Crystal structures of a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus: insights into an adapted mechanism of ligand binding.,Kumar P, Kesari P, Kokane S, Ghosh DK, Kumar P, Sharma AK FEBS J. 2019 May 7. doi: 10.1111/febs.14921. PMID:31063259^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumar P, Kesari P, Kokane S, Ghosh DK, Kumar P, Sharma AK. Crystal structures of a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus: insights into an adapted mechanism of ligand binding. FEBS J. 2019 May 7. doi: 10.1111/febs.14921. PMID:31063259 doi:http://dx.doi.org/10.1111/febs.14921

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6a80"

@@ Line 3: / Line 3: @@
 <StructureSection load='6a80' size='340' side='right'caption='[[6a80]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6a80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A80 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6a80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus_str._psy62 Candidatus Liberibacter asiaticus str. psy62]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A80 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a80 OCA], [http://pdbe.org/6a80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a80 RCSB], [http://www.ebi.ac.uk/pdbsum/6a80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a80 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a80 OCA], [https://pdbe.org/6a80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a80 RCSB], [https://www.ebi.ac.uk/pdbsum/6a80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a80 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C6XGT2_LIBAP C6XGT2_LIBAP]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Candidatus Liberibacter asiaticus str. psy62]]
 [[Category: Large Structures]]
-[[Category: Ghosh, D K]]
+[[Category: Ghosh DK]]
-[[Category: Kesari, P]]
+[[Category: Kesari P]]
-[[Category: Kumar, P]]
+[[Category: Kumar P]]
-[[Category: Sharma, A K]]
+[[Category: Sharma AK]]
-[[Category: Candidatus liberibacter asiaticus]]
-[[Category: Periplasmic domain]]
-[[Category: Putative amino acid binding]]
-[[Category: Transport protein]]

6a80

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Current revision

Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with cystine

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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