1eb4
From Proteopedia
(New page: 200px<br /> <applet load="1eb4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eb4, resolution 2.0Å" /> '''HISTIDINE AMMONIA-LY...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1EB4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB4 OCA]]. | + | 1EB4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB4 OCA]]. |
==Reference== | ==Reference== | ||
Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase., Baedeker M, Schulz GE, Structure. 2002 Jan;10(1):61-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11796111 11796111] | Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase., Baedeker M, Schulz GE, Structure. 2002 Jan;10(1):61-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11796111 11796111] | ||
| + | [[Category: Histidine ammonia-lyase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:19:08 2007'' |
Revision as of 09:14, 30 October 2007
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HISTIDINE AMMONIA-LYASE (HAL) MUTANT F329A FROM PSEUDOMONAS PUTIDA
Overview
Histidine ammonia-lyase requires a 4-methylidene-imidazole-5-one group, (MIO) that is produced autocatalytically by a cyclization and dehydration, step in a 3-residue loop of the polypeptide. The crystal structures of, three mutants have been established. Two mutants were inactive and failed, to form MIO, but remained unchanged elsewhere. The third mutant showed, very low activity and formed MIO, although it differed from an MIO-less, mutant only by an additional 329-C(beta) atom. This atom forms one, constraint during MIO formation, the other being the strongly connected, Asp145. An exploration of the conformational space of the MIO-forming loop, showed that the cyclization is probably enforced by a mechanic compression, in a late stage of chain folding and is catalyzed by a ... [(full description)]
About this Structure
1EB4 is a [Single protein] structure of sequence from [Pseudomonas putida] with SO4 and GOL as [ligands]. Active as [Histidine ammonia-lyase], with EC number [4.3.1.3]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase., Baedeker M, Schulz GE, Structure. 2002 Jan;10(1):61-7. PMID:11796111
Page seeded by OCA on Tue Oct 30 11:19:08 2007
