2j58
From Proteopedia
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| - | [[Image:2j58. | + | [[Image:2j58.jpg|left|200px]]<br /><applet load="2j58" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j58" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2j58, resolution 2.25Å" /> | caption="2j58, resolution 2.25Å" /> | ||
'''THE STRUCTURE OF WZA'''<br /> | '''THE STRUCTURE OF WZA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J58 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, OCT and HEX as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2J58 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, OCT and HEX as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain H'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J58 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: membrane protein]] | [[Category: membrane protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:49:25 2007'' |
Revision as of 17:39, 18 December 2007
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THE STRUCTURE OF WZA
Overview
Many types of bacteria produce extracellular polysaccharides (EPSs). Some, are secreted polymers and show only limited association with the cell, surface, whereas others are firmly attached to the cell surface and form a, discrete structural layer, the capsule, which envelopes the cell and, allows the bacteria to evade or counteract the host immune system. EPSs, have critical roles in bacterial colonization of surfaces, such as, epithelia and medical implants; in addition some EPSs have important, industrial and biomedical applications in their own right. Here we, describe the 2.26 A resolution structure of the 340 kDa octamer of Wza, an, integral outer membrane lipoprotein, which is essential for group 1, capsule export in Escherichia coli. The transmembrane region is a novel, alpha-helical barrel. The bulk of the Wza structure is located in the, periplasm and comprises three novel domains forming a large central, cavity. Wza is open to the extracellular environment but closed to the, periplasm. We propose a route and mechanism for translocation of the, capsular polysaccharide. This work may provide insight into the export of, other large polar molecules such as DNA and proteins.
About this Structure
2J58 is a Single protein structure of sequence from Escherichia coli with SO4, OCT and HEX as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein., Dong C, Beis K, Nesper J, Brunkan-Lamontagne AL, Clarke BR, Whitfield C, Naismith JH, Nature. 2006 Nov 9;444(7116):226-9. Epub 2006 Nov 1. PMID:17086202
Page seeded by OCA on Tue Dec 18 19:49:25 2007
Categories: Escherichia coli | Single protein | Dong, C. | Naismith, J.H. | HEX | OCT | SO4 | Lipoprotein | Membrane protein
