User:Luis Andres Casavilca Ramirez/Sandbox 1
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | <scene name='81/817991/Pbucas13b_domains/2'>Text To Be Displayed</scene>==Introduction== | + | <scene name='81/817991/Hepn1_and_hepn2/1'>Text To Be Displayed</scene><scene name='81/817991/Pbucas13b_domains/2'>Text To Be Displayed</scene>==Introduction== |
<StructureSection load='6dtd' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='6dtd' size='340' side='right' caption='Caption for this structure' scene=''> | ||
PduCas13b is a class 2 type 4 from Prevotella buccae. As a member of this protein family, it uses a single effector complexed with a CRISPR-RNA to bind and cleave RNA with its twin HEPN domains, activating a general non-specific RNase activity that degrades any nearby transcripts.(1) However, PduCas13b is structurally and mechanically different from the other Cas13s, as it has a unique linear organization and different dynamics in target recognition.(2). | PduCas13b is a class 2 type 4 from Prevotella buccae. As a member of this protein family, it uses a single effector complexed with a CRISPR-RNA to bind and cleave RNA with its twin HEPN domains, activating a general non-specific RNase activity that degrades any nearby transcripts.(1) However, PduCas13b is structurally and mechanically different from the other Cas13s, as it has a unique linear organization and different dynamics in target recognition.(2). | ||
== Structure == | == Structure == | ||
| - | Though the overall conserved bilobed shape of many Class 2 effectors consisting of a recognition and nuclease lobe is present,(2,3) Cas13b has a unique domain organization even among Cas13s. (Fig.1). A crystal structure of PbuCas13b complexed with a 36-nt direct repeat sequence and a 5-nt spacer at 1.65 Å provides information of the <scene name='81/817991/Pbucas13b_domains/2'>domains’ three dimensional arrange</scene>. There are five domains within the effector structure: two HEPN domains (HEPN1 and HEPN2), two mainly helical domains (Helical-1 and Helical-2), and a Lid domain. In addition, crRNA also has a unique structure, since its direct repeat is at the 3’ end and not at the 5’ end as in the other Cas13s (Fig.1). | + | Though the overall conserved bilobed shape of many Class 2 effectors consisting of a recognition and nuclease lobe is present,(2,3) Cas13b has a unique domain organization even among Cas13s. (Fig.1). A crystal structure of PbuCas13b complexed with a 36-nt direct repeat sequence and a 5-nt spacer at 1.65 Å provides information of the <scene name='81/817991/Pbucas13b_domains/2'>domains’ three dimensional arrange</scene>. There are five domains within the effector structure: two HEPN domains (<scene name='81/817991/Hepn1_and_hepn2/1'>HEPN1 and HEPN2</scene>), two mainly helical domains (Helical-1 and Helical-2), and a Lid domain. In addition, crRNA also has a unique structure, since its direct repeat is at the 3’ end and not at the 5’ end as in the other Cas13s (Fig.1). |
== cnRNA and Targeting == | == cnRNA and Targeting == | ||
Revision as of 14:31, 14 June 2019
==Introduction==
| |||||||||||
