2j6a
From Proteopedia
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| - | [[Image:2j6a. | + | [[Image:2j6a.jpg|left|200px]]<br /><applet load="2j6a" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2j6a" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="2j6a, resolution 1.70Å" /> | caption="2j6a, resolution 1.70Å" /> | ||
'''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.'''<br /> | '''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 2J6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J6A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: translation termination]] | [[Category: translation termination]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:50:50 2007'' |
Revision as of 17:41, 18 December 2007
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CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.
Overview
Protein release factor eRF1 in Saccharomyces cerevisiae, in complex with, eRF3 and GTP, is methylated on a functionally crucial Gln residue by the, S-adenosylmethionine-dependent methyltransferase Ydr140w. Here we show, that eRF1 methylation, in addition to these previously characterized, components, requires a 15-kDa zinc-binding protein, Ynr046w. Co-expression, in Escherichia coli of Ynr046w and Ydr140w allows the latter to be, recovered in soluble form rather than as inclusion bodies, and the two, proteins co-purify on nickel-nitrilotriacetic acid chromatography when, Ydr140w alone carries a His tag. The crystal structure of Ynr046w has been, determined to 1.7 A resolution. It comprises a zinc-binding domain built, from both the N- and C-terminal sequences and an inserted domain, absent, from bacterial and archaeal orthologs of the protein, composed of three, alpha-helices. The active methyltransferase is the heterodimer, Ydr140w.Ynr046w, but when alone, both in solution and in crystals, Ynr046w, appears to be a homodimer. The Ynr046w eRF1 methyltransferase subunit is, shared by the tRNA methyltransferase Trm11p and probably by two other, enzymes containing a Rossman fold.
About this Structure
2J6A is a Single protein structure of sequence from Saccharomyces cerevisiae with ZN and EDO as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308
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