6k0a
From Proteopedia
(Difference between revisions)
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/RNP4_METJA RNP4_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00757]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP2_METJA RNP2_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00755]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP1_METJA RNP1_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00754]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP3_METJA RNP3_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00756]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RL7A_METJA RL7A_METJA]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. | [[http://www.uniprot.org/uniprot/RNP4_METJA RNP4_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00757]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP2_METJA RNP2_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00755]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP1_METJA RNP1_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00754]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RNP3_METJA RNP3_METJA]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00756]<ref>PMID:18558617</ref> <ref>PMID:21683084</ref> <ref>PMID:22298511</ref> [[http://www.uniprot.org/uniprot/RL7A_METJA RL7A_METJA]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ribonuclease P (RNase P) is an essential ribozyme responsible for tRNA 5' maturation. Here we report the cryo-EM structures of Methanocaldococcus jannaschii (Mja) RNase P holoenzyme alone and in complex with a tRNA substrate at resolutions of 4.6 A and 4.3 A, respectively. The structures reveal that the subunits of MjaRNase P are strung together to organize the holoenzyme in a dimeric conformation required for efficient catalysis. The structures also show that archaeal RNase P is a functional chimera of bacterial and eukaryal RNase Ps that possesses bacterial-like two RNA-based anchors and a eukaryal-like protein-aided stabilization mechanism. The 3'-RCCA sequence of tRNA, which is a key recognition element for bacterial RNase P, is dispensable for tRNA recognition by MjaRNase P. The overall organization of MjaRNase P, particularly within the active site, is similar to those of bacterial and eukaryal RNase Ps, suggesting a universal catalytic mechanism for all RNase Ps. | ||
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| + | Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme.,Wan F, Wang Q, Tan J, Tan M, Chen J, Shi S, Lan P, Wu J, Lei M Nat Commun. 2019 Jun 13;10(1):2617. doi: 10.1038/s41467-019-10496-3. PMID:31197137<ref>PMID:31197137</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6k0a" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 06:52, 3 July 2019
cryo-EM structure of an archaeal Ribonuclease P
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