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| | <StructureSection load='6fms' size='340' side='right'caption='[[6fms]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='6fms' size='340' side='right'caption='[[6fms]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fms]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FMS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FMS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fms]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FMS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5BV:(2R,3R)-3-(GLYCYLOXY)-2-METHYLNONANOIC+ACID'>5BV</scene>, <scene name='pdbligand=ALO:ALLO-THREONINE'>ALO</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene>, <scene name='pdbligand=MLE:N-METHYLLEUCINE'>MLE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5BV:(2R,3R)-3-(GLYCYLOXY)-2-METHYLNONANOIC+ACID'>5BV</scene>, <scene name='pdbligand=ALO:ALLO-THREONINE'>ALO</scene>, <scene name='pdbligand=IIL:ISO-ISOLEUCINE'>IIL</scene>, <scene name='pdbligand=MLE:N-METHYLLEUCINE'>MLE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lspA, ls, PA4559 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fms OCA], [https://pdbe.org/6fms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fms RCSB], [https://www.ebi.ac.uk/pdbsum/6fms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fms ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal_peptidase_II Signal peptidase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.36 3.4.23.36] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fms OCA], [http://pdbe.org/6fms PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fms RCSB], [http://www.ebi.ac.uk/pdbsum/6fms PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fms ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LSPA_PSEAE LSPA_PSEAE]] This protein specifically catalyzes the removal of signal peptides from prolipoproteins. | + | [https://www.uniprot.org/uniprot/LSPA_PSEAE LSPA_PSEAE] This protein specifically catalyzes the removal of signal peptides from prolipoproteins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Signal peptidase II]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Basu, S]] | + | [[Category: Basu S]] |
| - | [[Category: Caffrey, M]] | + | [[Category: Caffrey M]] |
| - | [[Category: Diederichs, K]] | + | [[Category: Diederichs K]] |
| - | [[Category: Howe, N]] | + | [[Category: Howe N]] |
| - | [[Category: Huang, C Y]] | + | [[Category: Huang C-Y]] |
| - | [[Category: Olieric, V]] | + | [[Category: Olieric V]] |
| - | [[Category: Panepucci, E]] | + | [[Category: Panepucci E]] |
| - | [[Category: Vogeley, L]] | + | [[Category: Vogeley L]] |
| - | [[Category: Wang, M]] | + | [[Category: Wang M]] |
| - | [[Category: Warshamanage, R]] | + | [[Category: Warshamanage R]] |
| - | [[Category: Weinert, T]] | + | [[Category: Weinert T]] |
| - | [[Category: Experimental phasing]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: In meso crystallization]]
| + | |
| - | [[Category: In situ diffraction data collection]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Membrane protein structure]]
| + | |
| - | [[Category: Serial crystallography]]
| + | |
| Structural highlights
6fms is a 8 chain structure with sequence from Pseudomonas aeruginosa PAO1 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 3Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
LSPA_PSEAE This protein specifically catalyzes the removal of signal peptides from prolipoproteins.
Publication Abstract from PubMed
De novo membrane protein structure determination is often limited by the availability of large crystals and the difficulties in obtaining accurate diffraction data for experimental phasing. Here we present a method that combines in situ serial crystallography with de novo phasing for fast, efficient membrane protein structure determination. The method enables systematic diffraction screening and rapid data collection from hundreds of microcrystals in in meso crystallization wells without the need for direct crystal harvesting. The requisite data quality for experimental phasing is achieved by accumulating diffraction signals from isomorphous crystals identified post-data collection. The method works in all experimental phasing scenarios and is particularly attractive with fragile, weakly diffracting microcrystals. The automated serial data collection approach can be readily adopted at most microfocus macromolecular crystallography beamlines.
In situ serial crystallography for rapid de novo membrane protein structure determination.,Huang CY, Olieric V, Howe N, Warshamanage R, Weinert T, Panepucci E, Vogeley L, Basu S, Diederichs K, Caffrey M, Wang M Commun Biol. 2018 Aug 27;1:124. doi: 10.1038/s42003-018-0123-6. eCollection 2018. PMID:30272004[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Huang CY, Olieric V, Howe N, Warshamanage R, Weinert T, Panepucci E, Vogeley L, Basu S, Diederichs K, Caffrey M, Wang M. In situ serial crystallography for rapid de novo membrane protein structure determination. Commun Biol. 2018 Aug 27;1:124. doi: 10.1038/s42003-018-0123-6. eCollection 2018. PMID:30272004 doi:http://dx.doi.org/10.1038/s42003-018-0123-6
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