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| | <StructureSection load='4zgp' size='340' side='right'caption='[[4zgp]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='4zgp' size='340' side='right'caption='[[4zgp]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zgp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZGP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zgp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZGP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zgo|4zgo]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgp OCA], [https://pdbe.org/4zgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zgp RCSB], [https://www.ebi.ac.uk/pdbsum/4zgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgp ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cdc123, SPAP27G11.03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgp OCA], [http://pdbe.org/4zgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zgp RCSB], [http://www.ebi.ac.uk/pdbsum/4zgp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zgp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO]] Regulates the cell cycle in a nutrient dependent manner. | + | [https://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO] Regulates the cell cycle in a nutrient dependent manner. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cbs 356]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Arnold, L]] | + | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: Dubiez, E]] | + | [[Category: Arnold L]] |
| - | [[Category: Mechulam, Y]] | + | [[Category: Dubiez E]] |
| - | [[Category: Panvert, M]] | + | [[Category: Mechulam Y]] |
| - | [[Category: Perez, J]] | + | [[Category: Panvert M]] |
| - | [[Category: Schmitt, E]] | + | [[Category: Perez J]] |
| - | [[Category: Seufert, W]] | + | [[Category: Schmitt E]] |
| - | [[Category: Atp-grap fold]]
| + | [[Category: Seufert W]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Eif2]]
| + | |
| Structural highlights
Function
CD123_SCHPO Regulates the cell cycle in a nutrient dependent manner.
Publication Abstract from PubMed
Eukaryotic initiation factor 2 (eIF2), a heterotrimeric guanosine triphosphatase, has a central role in protein biosynthesis by supplying methionylated initiator tRNA to the ribosomal translation initiation complex and by serving as a target for translational control in response to stress. Recent work identified a novel step indispensable for eIF2 function: assembly of eIF2 from its three subunits by the cell proliferation protein Cdc123. We report the first crystal structure of a Cdc123 representative, that from Schizosaccharomyces pombe, both isolated and bound to domain III of Saccharomyces cerevisiae eIF2gamma. The structures show that Cdc123 resembles enzymes of the ATP-grasp family. Indeed, Cdc123 binds ATP-Mg(2+), and conserved residues contacting ATP-Mg(2+) are essential for Cdc123 to support eIF2 assembly and cell viability. A docking of eIF2alphagamma onto Cdc123, combined with genetic and biochemical experiments, allows us to propose a model explaining how Cdc123 participates in the biogenesis of eIF2 through facilitating assembly of eIF2gamma to eIF2alpha.
Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features.,Panvert M, Dubiez E, Arnold L, Perez J, Mechulam Y, Seufert W, Schmitt E Structure. 2015 Sep 1;23(9):1596-608. doi: 10.1016/j.str.2015.06.014. Epub 2015, Jul 23. PMID:26211610[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Panvert M, Dubiez E, Arnold L, Perez J, Mechulam Y, Seufert W, Schmitt E. Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Structure. 2015 Sep 1;23(9):1596-608. doi: 10.1016/j.str.2015.06.014. Epub 2015, Jul 23. PMID:26211610 doi:http://dx.doi.org/10.1016/j.str.2015.06.014
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