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| | <StructureSection load='4xbh' size='340' side='right'caption='[[4xbh]], [[Resolution|resolution]] 2.11Å' scene=''> | | <StructureSection load='4xbh' size='340' side='right'caption='[[4xbh]], [[Resolution|resolution]] 2.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xbh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XBH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xbh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XBH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MME ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xbh OCA], [https://pdbe.org/4xbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xbh RCSB], [https://www.ebi.ac.uk/pdbsum/4xbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xbh ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neprilysin Neprilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.11 3.4.24.11] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xbh OCA], [http://pdbe.org/4xbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xbh RCSB], [http://www.ebi.ac.uk/pdbsum/4xbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xbh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NEP_RABIT NEP_RABIT]] Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.[UniProtKB:P08473] | + | [https://www.uniprot.org/uniprot/NEP_RABIT NEP_RABIT] Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.[UniProtKB:P08473] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: European rabbit]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Neprilysin]] | + | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Grochulski, P]] | + | [[Category: Grochulski P]] |
| - | [[Category: Labiuk, S L]] | + | [[Category: Labiuk SL]] |
| - | [[Category: Sygusch, J]] | + | [[Category: Sygusch J]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Neutral endopeptidase]]
| + | |
| - | [[Category: Proteinase]]
| + | |
| - | [[Category: Zn-dependent]]
| + | |
| Structural highlights
Function
NEP_RABIT Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.[UniProtKB:P08473]
Publication Abstract from PubMed
Neutral endopeptidase (neprilysin; NEP) is a proteinase that cleaves a wide variety of peptides and has been implicated in Alzheimer's disease, cardiovascular conditions, arthritis and other inflammatory diseases. The structure of the soluble extracellular domain (residues 55-750) of rabbit neprilysin was solved both in its native form at 2.1 A resolution, and bound to the inhibitors phosphoramidon and thiorphan at 2.8 and 3.0 A resolution, respectively. Consistent with the extracellular domain of human neprilysin, the structure reveals a large central cavity which contains the active site and the location for inhibitor binding.
Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan.,Labiuk SL, Sygusch J, Grochulski P Acta Crystallogr F Struct Biol Commun. 2019 Jun 1;75(Pt 6):405-411. doi:, 10.1107/S2053230X19006046. Epub 2019 May 10. PMID:31204686[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Labiuk SL, Sygusch J, Grochulski P. Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan. Acta Crystallogr F Struct Biol Commun. 2019 Jun 1;75(Pt 6):405-411. doi:, 10.1107/S2053230X19006046. Epub 2019 May 10. PMID:31204686 doi:http://dx.doi.org/10.1107/S2053230X19006046
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