5a23

Structural highlights

Function

LPAKS_PSEAE Alkylsulfatase that cleaves both short and medium-length alkylsulfates, including the widely used detergent sodium dodecyl sulfate (SDS) (PubMed:16684886). Acts in vitro on primary alkylsulfates such as decyl sulfate, octyl sulfate and hexyl sulfate, but not on sulfated sugars and arylsulfates (PubMed:16684886, PubMed:23061549). The hydrolysis of SDS allows the bacterium to use SDS as a sole carbon or sulfur source (PubMed:16684886). In addition, contributes to the in vivo virulence of P.aeruginosa by playing a central role in the degradation of mucin, a glycoprotein that is the primary component of the mucus overlaying the host epithelial tissues (PubMed:31907968). Plays a role in the degradation of both protein and carbohydrate moieties in mucin via the release of sulfate from mucin, D-galactose 6-sulfate (Gal-6S) and N-acetyl-D-glucosamine 6-sulfate (GlcNAc-6S) (PubMed:31907968).^{[1] [2] [3]}

References

1. ↑ Hagelueken G, Adams TM, Wiehlmann L, Widow U, Kolmar H, Tummler B, Heinz DW, Schubert WD. The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7631-6. Epub 2006 May 9. PMID:16684886
2. ↑ Knaus T, Schober M, Kepplinger B, Faccinelli M, Pitzer J, Faber K, Macheroux P, Wagner U. Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates. FEBS J. 2012 Dec;279(23):4374-84. doi: 10.1111/febs.12027. Epub 2012 Nov 7. PMID:23061549 doi:http://dx.doi.org/10.1111/febs.12027
3. ↑ Kida Y, Yamamoto T, Kuwano K. SdsA1, a secreted sulfatase, contributes to the in vivo virulence of Pseudomonas aeruginosa in mice. Microbiol Immunol. 2020 Apr;64(4):280-295. PMID:31907968 doi:10.1111/1348-0421.12772