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| | <StructureSection load='4zn1' size='340' side='right'caption='[[4zn1]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='4zn1' size='340' side='right'caption='[[4zn1]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4zn1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZN1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4zn1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZN1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zn3|4zn3]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zn1 OCA], [https://pdbe.org/4zn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zn1 RCSB], [https://www.ebi.ac.uk/pdbsum/4zn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zn1 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spt5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), spt4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zn1 OCA], [http://pdbe.org/4zn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zn1 RCSB], [http://www.ebi.ac.uk/pdbsum/4zn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zn1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPT5_METJA SPT5_METJA]] Stimulates transcription elongation.[HAMAP-Rule:MF_00950]<ref>PMID:20197319</ref> [[http://www.uniprot.org/uniprot/SPT4_METJA SPT4_METJA]] Stimulates transcription elongation.[HAMAP-Rule:MF_00949]<ref>PMID:20197319</ref> | + | [https://www.uniprot.org/uniprot/SPT5_METJA SPT5_METJA] Stimulates transcription elongation.[HAMAP-Rule:MF_00950]<ref>PMID:20197319</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Metja]] | + | [[Category: Methanocaldococcus jannaschii DSM 2661]] |
| - | [[Category: Gao, Y X]] | + | [[Category: Gao YX]] |
| - | [[Category: Guo, G R]] | + | [[Category: Guo GR]] |
| - | [[Category: Niu, L W]] | + | [[Category: Niu LW]] |
| - | [[Category: Teng, M K]] | + | [[Category: Teng MK]] |
| - | [[Category: Zhou, H H]] | + | [[Category: Zhou HH]] |
| - | [[Category: Zhu, Z L]] | + | [[Category: Zhu ZL]] |
| - | [[Category: Protein-protein complex]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
SPT5_METJA Stimulates transcription elongation.[HAMAP-Rule:MF_00950][1]
Publication Abstract from PubMed
Spt5 (NusG in bacteria) is the only RNA polymerase-associated factor known to be conserved in all three domains of life. In archaea and eukaryotes, Spt5 associates with Spt4, an elongation factor that is absent in bacteria, to form a functional heterodimeric complex. Previous studies suggest that the Spt4:Spt5 complex interacts directly with DNA at the double-stranded DNA exit tunnel of RNA polymerase to regulate gene transcription. In this study, the DNA-binding ability of Spt4:Spt5 from the archaeon Methanocaldococcus jannaschii was confirmed via nuclear magnetic resonance chemical shift perturbation and fluorescence polarization assays. Crystallographic analysis of the full-length MjSpt4:Spt5 revealed two distinct conformations of the C-terminal KOW domain of Spt5. A similar alkaline region was found on the Spt4:Spt5 surface in both crystal forms, and identified as double-stranded DNA binding patch through mutagenesis-fluorescence polarization assays. Based on these structural and biochemical data, the Spt4:Spt5-DNA binding model was built for the first time.
Structural and biochemical insights into the DNA-binding mode of MjSpt4p:Spt5 complex at the exit tunnel of RNAPII.,Guo G, Gao Y, Zhu Z, Zhao D, Liu Z, Zhou H, Niu L, Teng M J Struct Biol. 2015 Dec;192(3):418-25. doi: 10.1016/j.jsb.2015.09.023. Epub 2015 , Oct 14. PMID:26433031[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hirtreiter A, Damsma GE, Cheung AC, Klose D, Grohmann D, Vojnic E, Martin AC, Cramer P, Werner F. Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif. Nucleic Acids Res. 2010 Jul 1;38(12):4040-51. Epub 2010 Mar 2. PMID:20197319 doi:10.1093/nar/gkq135
- ↑ Guo G, Gao Y, Zhu Z, Zhao D, Liu Z, Zhou H, Niu L, Teng M. Structural and biochemical insights into the DNA-binding mode of MjSpt4p:Spt5 complex at the exit tunnel of RNAPII. J Struct Biol. 2015 Dec;192(3):418-25. doi: 10.1016/j.jsb.2015.09.023. Epub 2015 , Oct 14. PMID:26433031 doi:http://dx.doi.org/10.1016/j.jsb.2015.09.023
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