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1gt0

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Revision as of 06:40, 24 July 2019

Crystal structure of a POU/HMG/DNA ternary complex

Structural highlights

1gt0 is a 4 chain structure with sequence from Human and Lk3 transgenic mice. The April 2009 RCSB PDB Molecule of the Month feature on Oct and Sox Transcription Factors by David Goodsell is 10.2210/rcsb_pdb/mom_2009_4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1cqt, 1e3o, 1hf0, 1oct, 1pog, 1pou
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SOX2_MOUSE] Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).^[1] ^[2] [PO2F1_HUMAN] Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Members of the POU and SOX transcription factor families exemplify the partnerships established between various transcriptional regulators during early embryonic development. Although functional cooperativity between key regulator proteins is pivotal for milestone decisions in mammalian development, little is known about the underlying molecular mechanisms. In this study, we focus on two transcription factors, Oct4 and Sox2, as their combination on DNA is considered to direct the establishment of the first three lineages in the mammalian embryo. Using experimental high-resolution structure determination, followed by model building and experimental validation, we found that Oct4 and Sox2 were able to dimerize onto DNA in distinct conformational arrangements. We demonstrate that the DNA enhancer region of their target genes is responsible for the correct spatial alignment of glue-like interaction domains on their surface. Interestingly, these surfaces frequently have redundant functions and are instrumental in recruiting various interacting protein partners.

Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.,Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M Genes Dev. 2003 Aug 15;17(16):2048-59. PMID:12923055^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tsuruzoe S, Ishihara K, Uchimura Y, Watanabe S, Sekita Y, Aoto T, Saitoh H, Yuasa Y, Niwa H, Kawasuji M, Baba H, Nakao M. Inhibition of DNA binding of Sox2 by the SUMO conjugation. Biochem Biophys Res Commun. 2006 Dec 29;351(4):920-6. Epub 2006 Nov 2. PMID:17097055 doi:http://dx.doi.org/S0006-291X(06)02390-4
↑ Andreu-Agullo C, Maurin T, Thompson CB, Lai EC. Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2. Nature. 2011 Dec 25;481(7380):195-8. doi: 10.1038/nature10712. PMID:22198669 doi:http://dx.doi.org/10.1038/nature10712
↑ Segil N, Roberts SB, Heintz N. Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity. Science. 1991 Dec 20;254(5039):1814-6. PMID:1684878
↑ Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M. Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers. Genes Dev. 2003 Aug 15;17(16):2048-59. PMID:12923055 doi:http://dx.doi.org/10.1101/gad.269303

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gt0"

@@ Line 3: / Line 3: @@
 <StructureSection load='1gt0' size='340' side='right'caption='[[1gt0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1gt0]] is a 4 chain structure. The April 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Oct and Sox Transcription Factors''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_4 10.2210/rcsb_pdb/mom_2009_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GT0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1gt0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. The April 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Oct and Sox Transcription Factors''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_4 10.2210/rcsb_pdb/mom_2009_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GT0 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cqt|1cqt]], [[1e3o|1e3o]], [[1hf0|1hf0]], [[1oct|1oct]], [[1pog|1pog]], [[1pou|1pou]]</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gt0 OCA], [http://pdbe.org/1gt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gt0 RCSB], [http://www.ebi.ac.uk/pdbsum/1gt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gt0 ProSAT]</span></td></tr>
@@ Line 35: / Line 35: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Large Structures]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Oct and Sox Transcription Factors]]
 [[Category: RCSB PDB Molecule of the Month]]

1gt0

From Proteopedia

Revision as of 06:40, 24 July 2019

Crystal structure of a POU/HMG/DNA ternary complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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