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4zn3

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Revision as of 07:34, 18 May 2023

Crystal Structure of MjSpt4:Spt5 complex conformation B

Structural highlights

4zn3 is a 2 chain structure with sequence from Methanocaldococcus jannaschii DSM 2661. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPT5_METJA Stimulates transcription elongation.[HAMAP-Rule:MF_00950]^[1]

Publication Abstract from PubMed

Spt5 (NusG in bacteria) is the only RNA polymerase-associated factor known to be conserved in all three domains of life. In archaea and eukaryotes, Spt5 associates with Spt4, an elongation factor that is absent in bacteria, to form a functional heterodimeric complex. Previous studies suggest that the Spt4:Spt5 complex interacts directly with DNA at the double-stranded DNA exit tunnel of RNA polymerase to regulate gene transcription. In this study, the DNA-binding ability of Spt4:Spt5 from the archaeon Methanocaldococcus jannaschii was confirmed via nuclear magnetic resonance chemical shift perturbation and fluorescence polarization assays. Crystallographic analysis of the full-length MjSpt4:Spt5 revealed two distinct conformations of the C-terminal KOW domain of Spt5. A similar alkaline region was found on the Spt4:Spt5 surface in both crystal forms, and identified as double-stranded DNA binding patch through mutagenesis-fluorescence polarization assays. Based on these structural and biochemical data, the Spt4:Spt5-DNA binding model was built for the first time.

Structural and biochemical insights into the DNA-binding mode of MjSpt4p:Spt5 complex at the exit tunnel of RNAPII.,Guo G, Gao Y, Zhu Z, Zhao D, Liu Z, Zhou H, Niu L, Teng M J Struct Biol. 2015 Dec;192(3):418-25. doi: 10.1016/j.jsb.2015.09.023. Epub 2015 , Oct 14. PMID:26433031^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hirtreiter A, Damsma GE, Cheung AC, Klose D, Grohmann D, Vojnic E, Martin AC, Cramer P, Werner F. Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif. Nucleic Acids Res. 2010 Jul 1;38(12):4040-51. Epub 2010 Mar 2. PMID:20197319 doi:10.1093/nar/gkq135
↑ Guo G, Gao Y, Zhu Z, Zhao D, Liu Z, Zhou H, Niu L, Teng M. Structural and biochemical insights into the DNA-binding mode of MjSpt4p:Spt5 complex at the exit tunnel of RNAPII. J Struct Biol. 2015 Dec;192(3):418-25. doi: 10.1016/j.jsb.2015.09.023. Epub 2015 , Oct 14. PMID:26433031 doi:http://dx.doi.org/10.1016/j.jsb.2015.09.023

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4zn3"

@@ Line 3: / Line 3: @@
 <StructureSection load='4zn3' size='340' side='right'caption='[[4zn3]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zn3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZN3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZN3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zn3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZN3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zn1|4zn1]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zn3 OCA], [https://pdbe.org/4zn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zn3 RCSB], [https://www.ebi.ac.uk/pdbsum/4zn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zn3 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spt5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA]), spt4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zn3 OCA], [http://pdbe.org/4zn3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zn3 RCSB], [http://www.ebi.ac.uk/pdbsum/4zn3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zn3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SPT5_METJA SPT5_METJA]] Stimulates transcription elongation.[HAMAP-Rule:MF_00950]<ref>PMID:20197319</ref>  [[http://www.uniprot.org/uniprot/SPT4_METJA SPT4_METJA]] Stimulates transcription elongation.[HAMAP-Rule:MF_00949]<ref>PMID:20197319</ref>
+[https://www.uniprot.org/uniprot/SPT5_METJA SPT5_METJA] Stimulates transcription elongation.[HAMAP-Rule:MF_00950]<ref>PMID:20197319</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 28: / Line 26: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Metja]]
+[[Category: Methanocaldococcus jannaschii DSM 2661]]
-[[Category: Gao, Y X]]
+[[Category: Gao YX]]
-[[Category: Guo, G R]]
+[[Category: Guo GR]]
-[[Category: Niu, L W]]
+[[Category: Niu LW]]
-[[Category: Teng, M K]]
+[[Category: Teng MK]]
-[[Category: Zhou, H H]]
+[[Category: Zhou HH]]
-[[Category: Zhu, Z L]]
+[[Category: Zhu ZL]]
-[[Category: Protein-protein complex]]
-[[Category: Transcription]]

4zn3

From Proteopedia

Revision as of 07:34, 18 May 2023

Crystal Structure of MjSpt4:Spt5 complex conformation B

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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