Journal:Acta Cryst D:S2059798319009008
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In the two test proteins, the <scene name='81/819238/Cv/2'>Orf11 NTD</scene> from ''Staphylococcus'' phage P68 and <scene name='81/819238/Cv/3'>Hen Egg White Lysozyme</scene>, the rMMS technique in the presence of I3C generated numerous new conditions where derivatized crystals could grow. The I3C molecules in the lattice were sufficient to completely solve the structure using anomalous phasing methods. | In the two test proteins, the <scene name='81/819238/Cv/2'>Orf11 NTD</scene> from ''Staphylococcus'' phage P68 and <scene name='81/819238/Cv/3'>Hen Egg White Lysozyme</scene>, the rMMS technique in the presence of I3C generated numerous new conditions where derivatized crystals could grow. The I3C molecules in the lattice were sufficient to completely solve the structure using anomalous phasing methods. | ||
| - | I3C was present in the tested crystals in the rMMS screens containing I3C, where they often sit at the junctions between protein molecules in the lattice. These bridging contacts could improve the crystal lattice or generate new conditions where crystals can grow. | + | I3C was present in the tested crystals in the rMMS screens containing I3C, where they often sit at the junctions between protein molecules in the lattice. These bridging contacts could improve the crystal lattice or generate new conditions where crystals can grow. |
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| + | I3C can mediate contacts between protein molecules in a crystal: | ||
| + | *<scene name='81/819238/Cv/5'>One I3C molecule from the lysozyme crystal</scene>. | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 12:47, 26 June 2019
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