6a6x
From Proteopedia
(Difference between revisions)
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<StructureSection load='6a6x' size='340' side='right'caption='[[6a6x]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='6a6x' size='340' side='right'caption='[[6a6x]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6a6x]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A6X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6a6x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A6X FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a6x OCA], [http://pdbe.org/6a6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a6x RCSB], [http://www.ebi.ac.uk/pdbsum/6a6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a6x ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a6x OCA], [http://pdbe.org/6a6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a6x RCSB], [http://www.ebi.ac.uk/pdbsum/6a6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a6x ProSAT]</span></td></tr> | ||
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/MAZF7_MYCTU MAZF7_MYCTU]] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an endoribonuclease (By similarity).[UniProtKB:P9WIH9]<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> [[http://www.uniprot.org/uniprot/MAZE7_MYCTU MAZE7_MYCTU]] Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli but not in M.smegmatis neutralizes the effect of cognate toxin MazF7.<ref>PMID:19016878</ref> | [[http://www.uniprot.org/uniprot/MAZF7_MYCTU MAZF7_MYCTU]] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an endoribonuclease (By similarity).[UniProtKB:P9WIH9]<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> [[http://www.uniprot.org/uniprot/MAZE7_MYCTU MAZE7_MYCTU]] Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli but not in M.smegmatis neutralizes the effect of cognate toxin MazF7.<ref>PMID:19016878</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Toxin-antitoxin (TA) modules widely exist in bacteria, and their activities are associated with the persister phenotype of the pathogen Mycobacterium tuberculosis ( M. tb). M. tb causes tuberculosis, a contagious and severe airborne disease. There are 10 MazEF TA systems in M. tb that play important roles in stress adaptation. How the antitoxins antagonize toxins in M. tb or how the 10 TA systems crosstalk to each other are of interest, but the detailed molecular mechanisms are largely unclear. MazEF-mt9 is a unique member among the MazEF family due to its tRNase activity, which is usually carried out by the VapC toxins. Here, we present the cocrystal structure of the MazEF-mt9 complex at 2.7 A. By characterizing the association mode between the TA pairs through various techniques, we found that MazF-mt9 bound not only its cognate antitoxin but also the noncognate antitoxin MazE-mt1, a phenomenon that could be also observed in vivo. Based on our structural and biochemical work, we propose that the cognate and heterologous interactions among different TA systems work together in vivo to relieve the toxicity of MazF-mt9 toward M. tb cells. | ||
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| + | Structural and Biochemical Characterization of the Cognate and Heterologous Interactions of the MazEF-mt9 TA System.,Chen R, Tu J, Tan Y, Cai X, Yang C, Deng X, Su B, Ma S, Liu X, Ma P, Du C, Xie W ACS Infect Dis. 2019 Jul 3. doi: 10.1021/acsinfecdis.9b00001. PMID:31267737<ref>PMID:31267737</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6a6x" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 08:32, 24 July 2019
The crystal structure of the Mtb MazE-MazF-mt9 complex
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