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5abb

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Revision as of 18:10, 6 March 2020

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion

5abb, resolution 8.00Å

Structural highlights

5abb is a 3 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[B7MCR5_ECO45] The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.[HAMAP-Rule:MF_01465][RuleBase:RU000537] [PRRG_PRB01] Light-driven proton pump that generates photothrophic energy. [B7MIW7_ECO45] Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.[HAMAP-Rule:MF_00422]

Publication Abstract from PubMed

The biogenesis of polytopic membrane proteins occurs co-translationally on ribosomes that are tightly bound to a membrane-embedded protein-conducting channel: the Sec-complex. The path that is followed by nascent proteins inside the ribosome and the Sec-complex is relatively well established; however, it is not clear what the fate of the N-terminal transmembrane domains (TMDs) of polytopic membrane proteins is when the C-terminal TMDs domains are not yet synthesized. Here, we present the sub-nanometer cryo-electron microscopy structure of an in vivo generated ribosome-SecY complex that carries a membrane insertion intermediate of proteorhodopsin (PR). The structure reveals a pre-opened Sec-complex and the first two TMDs of PR already outside the SecY complex directly in front of its proposed lateral gate. Thus, our structure is in agreement with positioning of N-terminal TMDs at the periphery of SecY, and in addition, it provides clues for the molecular mechanism underlying membrane protein topogenesis.

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion.,Bischoff L, Wickles S, Berninghausen O, van der Sluis EO, Beckmann R Nat Commun. 2014 Jun 10;5:4103. doi: 10.1038/ncomms5103. PMID:24912953^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bischoff L, Wickles S, Berninghausen O, van der Sluis EO, Beckmann R. Visualization of a polytopic membrane protein during SecY-mediated membrane insertion. Nat Commun. 2014 Jun 10;5:4103. doi: 10.1038/ncomms5103. PMID:24912953 doi:http://dx.doi.org/10.1038/ncomms5103

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5abb"

@@ Line 1: / Line 1: @@
 ==Visualization of a polytopic membrane protein during SecY-mediated membrane insertion==
-<StructureSection load='5abb' size='340' side='right'caption='[[5abb]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
+<SX load='5abb' size='340' side='right' viewer='molstar' caption='[[5abb]], [[Resolution|resolution]] 8.00&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5abb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ABB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ABB FirstGlance]. <br>
@@ Line 24: / Line 24: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Escherichia coli]]
 [[Category: Large Structures]]

5abb

From Proteopedia

Revision as of 18:10, 6 March 2020

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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