1uzw
From Proteopedia
(New page: 200px<br /> <applet load="1uzw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uzw, resolution 1.30Å" /> '''ISOPENICILLIN N SYN...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1UZW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2, SO4 and CDH as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZW OCA]]. | + | 1UZW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2, SO4 and CDH as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isopenicillin-N_synthase Isopenicillin-N synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZW OCA]]. |
==Reference== | ==Reference== | ||
Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase., Grummitt AR, Rutledge PJ, Clifton IJ, Baldwin JE, Biochem J. 2004 Sep 1;382(Pt 2):659-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15175003 15175003] | Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase., Grummitt AR, Rutledge PJ, Clifton IJ, Baldwin JE, Biochem J. 2004 Sep 1;382(Pt 2):659-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15175003 15175003] | ||
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
| + | [[Category: Isopenicillin-N synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Baldwin, J.E.]] | [[Category: Baldwin, J.E.]] | ||
| Line 27: | Line 28: | ||
[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:19:30 2007'' |
Revision as of 09:14, 30 October 2007
|
ISOPENICILLIN N SYNTHASE WITH L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-ISODEHYDROVALINE
Overview
Isopenicillin N synthase (IPNS) is a non-haem iron oxidase that catalyses, the formation of bicyclic isopenicillin N from, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). In this study we, report a novel activity for the iron of the IPNS active site, which, behaves as a Lewis acid to catalyse the elimination of HF from the, fluorinated substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-beta-fluorovaline (ACbetaFV)., X-Ray crystallographic studies of IPNS crystals grown anaerobically with, ACbetaFV reveal that the valinyl beta-fluorine is missing from the active, site region, and suggest the presence of the unsaturated tripeptide, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-isodehydrovaline in place of, substrate ACbetaFV. (19)F NMR studies confirm the release of ... [(full description)]
About this Structure
1UZW is a [Single protein] structure of sequence from [Emericella nidulans] with FE2, SO4 and CDH as [ligands]. Active as [Isopenicillin-N synthase], with EC number [1.21.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase., Grummitt AR, Rutledge PJ, Clifton IJ, Baldwin JE, Biochem J. 2004 Sep 1;382(Pt 2):659-66. PMID:15175003
Page seeded by OCA on Tue Oct 30 11:19:30 2007
