6a9s

From Proteopedia

(Difference between revisions)

Current revision

The crystal structure of vaccinia virus A26 (residues 1-397)

Structural highlights

6a9s is a 1 chain structure with sequence from Vaccinia virus WR. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.18Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PG153_VACCW Envelop protein that mediates acid-dependent endocytosis into host cells (PubMed:20538855). Plays an important role in endocytic entry of the virus by acting as an acid-sensitive membrane fusion suppressor (PubMed:22278246, PubMed:31220181). Low pH in host endosomes triggers conformational changes to allow de-repression of viral fusion complex activity and membrane fusion within vesicles (PubMed:31220181). Plays also a role in bridging the mature virion with structural protein OPG152.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Vaccinia mature virus requires A26 envelope protein to mediate acid-dependent endocytosis into HeLa cells in which we hypothesized that A26 protein functions as an acid-sensitive membrane fusion suppressor. Here, we provide evidence showing that N-terminal domain (aa1-75) of A26 protein is an acid-sensitive region that regulates membrane fusion. Crystal structure of A26 protein revealed that His48 and His53 are in close contact with Lys47, Arg57, His314 and Arg312, suggesting that at low pH these His-cation pairs could initiate conformational changes through protonation of His48 and His53 and subsequent electrostatic repulsion. All the A26 mutant mature viruses that interrupted His-cation pair interactions of His48 and His 53 indeed have lost virion infectivity. Isolation of revertant viruses revealed that second site mutations caused frame shifts and premature termination of A26 protein such that reverent viruses regained cell entry through plasma membrane fusion. Together, we conclude that viral A26 protein functions as an acid-sensitive fusion suppressor during vaccinia mature virus endocytosis.

Vaccinia viral A26 protein is a fusion suppressor of mature virus and triggers membrane fusion through conformational change at low pH.,Chang HW, Yang CH, Luo YC, Su BG, Cheng HY, Tung SY, Carillo KJD, Liao YT, Tzou DM, Wang HC, Chang W PLoS Pathog. 2019 Jun 20;15(6):e1007826. doi: 10.1371/journal.ppat.1007826., eCollection 2019 Jun. PMID:31220181^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chang SJ, Chang YX, Izmailyan R, Tang YL, Chang W. Vaccinia virus A25 and A26 proteins are fusion suppressors for mature virions and determine strain-specific virus entry pathways into HeLa, CHO-K1, and L cells. J Virol. 2010 Sep;84(17):8422-32. doi: 10.1128/JVI.00599-10. Epub 2010 Jun 10. PMID:20538855 doi:http://dx.doi.org/10.1128/JVI.00599-10
↑ Chang SJ, Shih AC, Tang YL, Chang W. Vaccinia mature virus fusion regulator A26 protein binds to A16 and G9 proteins of the viral entry fusion complex and dissociates from mature virions at low pH. J Virol. 2012 Apr;86(7):3809-18. PMID:22278246 doi:10.1128/JVI.06081-11
↑ Chang HW, Yang CH, Luo YC, Su BG, Cheng HY, Tung SY, Carillo KJD, Liao YT, Tzou DM, Wang HC, Chang W. Vaccinia viral A26 protein is a fusion suppressor of mature virus and triggers membrane fusion through conformational change at low pH. PLoS Pathog. 2019 Jun 20;15(6):e1007826. doi: 10.1371/journal.ppat.1007826., eCollection 2019 Jun. PMID:31220181 doi:http://dx.doi.org/10.1371/journal.ppat.1007826
↑ Chang HW, Yang CH, Luo YC, Su BG, Cheng HY, Tung SY, Carillo KJD, Liao YT, Tzou DM, Wang HC, Chang W. Vaccinia viral A26 protein is a fusion suppressor of mature virus and triggers membrane fusion through conformational change at low pH. PLoS Pathog. 2019 Jun 20;15(6):e1007826. doi: 10.1371/journal.ppat.1007826., eCollection 2019 Jun. PMID:31220181 doi:http://dx.doi.org/10.1371/journal.ppat.1007826

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6a9s"

@@ Line 3: / Line 3: @@
 <StructureSection load='6a9s' size='340' side='right'caption='[[6a9s]], [[Resolution|resolution]] 1.18&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6a9s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccw Vaccw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A9S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6a9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A9S FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.18&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VACWR149, A26L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10254 VACCW])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a9s OCA], [https://pdbe.org/6a9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a9s RCSB], [https://www.ebi.ac.uk/pdbsum/6a9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a9s ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a9s OCA], [http://pdbe.org/6a9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a9s RCSB], [http://www.ebi.ac.uk/pdbsum/6a9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a9s ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/A26_VACCW A26_VACCW]] Plays a role in bridging the mature virion with structural protein A25. Presence of protein A26 in the virion structurally prevents direct virus-cell fusion mechanism.<ref>PMID:20538855</ref>
+[https://www.uniprot.org/uniprot/PG153_VACCW PG153_VACCW] Envelop protein that mediates acid-dependent endocytosis into host cells (PubMed:20538855). Plays an important role in endocytic entry of the virus by acting as an acid-sensitive membrane fusion suppressor (PubMed:22278246, PubMed:31220181). Low pH in host endosomes triggers conformational changes to allow de-repression of viral fusion complex activity and membrane fusion within vesicles (PubMed:31220181). Plays also a role in bridging the mature virion with structural protein OPG152.<ref>PMID:20538855</ref> <ref>PMID:22278246</ref> <ref>PMID:31220181</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Vaccw]]
+[[Category: Vaccinia virus WR]]
-[[Category: Chang, W]]
+[[Category: Chang W]]
-[[Category: Ko, T Z]]
+[[Category: Ko TZ]]
-[[Category: Liao, Y T]]
+[[Category: Liao YT]]
-[[Category: Luo, Y C]]
+[[Category: Luo YC]]
-[[Category: Wang, H C]]
+[[Category: Wang HC]]
-[[Category: Acid sensitive]]
-[[Category: Vaccinia virus]]
-[[Category: Viral protein]]
-[[Category: Virus entry]]

6a9s

From Proteopedia

Current revision

The crystal structure of vaccinia virus A26 (residues 1-397)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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