This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3bc7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3bc7.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:3bc7.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3bc7| PDB=3bc7 | SCENE= }}
{{STRUCTURE_3bc7| PDB=3bc7 | SCENE= }}
-
'''The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site'''
+
===The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site===
-
==Overview==
+
<!--
-
Langerin is a type II transmembrane oligosaccharide receptor on Langerhans cells (LCs), a prominent subclass of dendritic cells (DCs) that mediate immune responses in epithelia and play a role in HIV degradation. Its extracellular moiety comprises a neck region with several heptad repeats and an exposed carboxy-terminal calcium-type carbohydrate-recognition domain (CRD). The CRD of human Langerin, which was expressed as a soluble protein in the periplasm of E. coli, was crystallized both alone and in the presence of two sugars, followed by X-ray analyses to resolutions of 2.5A for apo-Langerin and to 1.6A and 2.1A for the complexes with mannose and maltose, respectively. The fold of the Langerin CRD (dubbed LangA) resembles that of other typical C-type lectins such as DC-SIGN. However, especially in the long loop region (LLR), which is responsible for carbohydrate-binding, two additional secondary structure elements are present: a 3(10) helix and a small beta-sheet arising from the extended beta-strand 2, which enters into a hairpin and a new strand beta2'. Unexpectedly, the crystal structures in the presence of maltose and mannose reveal two sugar-binding sites. One is calcium-dependent and structurally conserved in the C-type lectin family whereas the second one represents a novel, calcium-independent type. Based on these data, a model for the binding of mannan, a component of many endogenous as well as viral glycoproteins, is proposed and the differences in binding behavior between Langerin and DC-SIGN with respect to the Lewis X carbohydrate antigen and its derivatives can be explained. Therefore, the crystal structure of LangA should be helpful for the development of new marker reagents selective for LCs and also of therapeutic compounds that may enhance the inhibitory role of Langerin towards HIV infection.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18061677}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18061677 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18061677}}
==About this Structure==
==About this Structure==
Line 39: Line 43:
[[Category: Sugar-binding]]
[[Category: Sugar-binding]]
[[Category: Transmembrane protein]]
[[Category: Transmembrane protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:32:43 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:47:19 2008''

Revision as of 19:47, 27 July 2008

Image:3bc7.png

Template:STRUCTURE 3bc7

The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site

Template:ABSTRACT PUBMED 18061677

About this Structure

3BC7 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site., Chatwell L, Holla A, Kaufer BB, Skerra A, Mol Immunol. 2008 Apr;45(7):1981-94. Epub 2007 Dec 3. PMID:18061677

Page seeded by OCA on Sun Jul 27 22:47:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bc7"
Personal tools