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6n1b

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Current revision

Crystal structure of an N-acetylgalactosamine deacetylase from F. plautii in complex with blood group B trisaccharide

Structural highlights

6n1b is a 1 chain structure with sequence from Flavonifractor plautii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.3Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G9YSP4_FLAPL

Publication Abstract from PubMed

Access to efficient enzymes that can convert A and B type red blood cells to 'universal' donor O would greatly increase the supply of blood for transfusions. Here we report the functional metagenomic screening of the human gut microbiome for enzymes that can remove the cognate A and B type sugar antigens. Among the genes encoded in our library of 19,500 expressed fosmids bearing gut bacterial DNA, we identify an enzyme pair from the obligate anaerobe Flavonifractor plautii that work in concert to efficiently convert the A antigen to the H antigen of O type blood, via a galactosamine intermediate. The X-ray structure of the N-acetylgalactosamine deacetylase reveals the active site and mechanism of the founding member of an esterase family. The galactosaminidase expands activities within the CAZy family GH36. Their ability to completely convert A to O of the same rhesus type at very low enzyme concentrations in whole blood will simplify their incorporation into blood transfusion practice, broadening blood supply.

An enzymatic pathway in the human gut microbiome that converts A to universal O type blood.,Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG Nat Microbiol. 2019 Jun 10. pii: 10.1038/s41564-019-0469-7. doi:, 10.1038/s41564-019-0469-7. PMID:31182795^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG. An enzymatic pathway in the human gut microbiome that converts A to universal O type blood. Nat Microbiol. 2019 Jun 10. pii: 10.1038/s41564-019-0469-7. doi:, 10.1038/s41564-019-0469-7. PMID:31182795 doi:http://dx.doi.org/10.1038/s41564-019-0469-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6n1b"

Categories: Flavonifractor plautii | Large Structures | Rahfeld P | Sim L | Withers SG

@@ Line 3: / Line 3: @@
 <StructureSection load='6n1b' size='340' side='right'caption='[[6n1b]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6n1b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_plauti"_(sic)_seguin_1928 "bacillus plauti" (sic) seguin 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N1B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6n1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavonifractor_plautii Flavonifractor plautii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N1B FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A4U99_12080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292800 "Bacillus plauti" (sic) Seguin 1928])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1b OCA], [http://pdbe.org/6n1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1b RCSB], [http://www.ebi.ac.uk/pdbsum/6n1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1b ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1b OCA], [https://pdbe.org/6n1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n1b RCSB], [https://www.ebi.ac.uk/pdbsum/6n1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1b ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/G9YSP4_FLAPL G9YSP4_FLAPL]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Flavonifractor plautii]]
 [[Category: Large Structures]]
-[[Category: Rahfeld, P]]
+[[Category: Rahfeld P]]
-[[Category: Sim, L]]
+[[Category: Sim L]]
-[[Category: Withers, S G]]
+[[Category: Withers SG]]
-[[Category: A blood antigen]]
-[[Category: Cazy]]
-[[Category: Hydrolase]]
-[[Category: N-acetylgalactosamine deacetylase]]

6n1b

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Current revision

Crystal structure of an N-acetylgalactosamine deacetylase from F. plautii in complex with blood group B trisaccharide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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